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Fatty acids resources:

Fatty acids research abs 1 || Fatty acids research abs 2 || Fatty acids research abs 3 || Fatty acids research abs 4







Mol Biochem Parasitol. 2002 Aug 28;123(2):85-94.
Functional characterization of the acyl carrier protein (PfACP) and beta-ketoacyl ACP synthase III (PfKASIII) from Plasmodium falciparum.

Waters NC, Kopydlowski KM, Guszczynski T, Wei L, Sellers P, Ferlan JT, Lee PJ, Li Z, Woodard CL, Shallom S, Gardner MJ, Prigge ST.

Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD 20910-5100, USA.

The genome of the malaria parasite, Plasmodium falciparum, appears to contain the proteins necessary for a Type II dissociated fatty acid biosynthetic system. Here we report the functional characterization of two proteins from this system. Purified recombinant acyl carrier protein (ACP) and beta-ketoacyl-ACP synthase III (KASIII) from P. falciparum are soluble and active in a truncated form. Malarial ACP is activated by the addition of a 4'-phosphopantetheine prosthetic group derived from coenzyme A, generating holo-PfACP. Holo-PfACP is an effective substrate for the transacylase activity of PfKASIII, but substitution of a key active site cysteine in PfKASIII to alanine or serine abolishes enzymatic activity. During the schizont stage of parasite development, there is a significant up-regulation of the mRNAs corresponding to these proteins, indicating an important metabolic requirement for fatty acids during this stage. 2002 Elsevier Science B.V.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12270624&dopt=Abstract



Toxicol Lett. 2002 Oct 5;135(3):209-17.
Assessment of the ability of the antioxidant cocktail-derived from fermentation of plants with effective microorganisms (EM-X) to modulate oxidative damage in the kidney and liver of rats in vivo: studies upon the profile of poly- and mono-unsaturated fatty acids.

Aruoma OI, Deiana M, Rosa A, Casu V, Piga R, Peccagnini S, Dessi MA, Ke B, Liang YF, Higa T.

Division of Neuroscience and Psychological Medicine, Department of Neuroinflammation, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Charing Cross Hospital Campus, Fulham Palace Road, London W6 8RF, UK. o.aruomc.ac.uk

The antioxidant cocktail EM-X derived from ferment of unpolished rice, papaya and sea weeds with effective microorganisms (EM) of lactic acid bacteria, yeast, and photosynthetic bacteria is widely available in South-East Asia. Oral administration of a EM-X to rats for 7 days inhibited the ferric-nitrilotriacetic acid (Fe-NTA)-dependent oxidation of fatty acids with protections directed towards docosahexanoic, arachidonic, docosapentanenoic acids, oleic, linoleic and eicosadieonoic acids in the liver and kidney. But only the protections of oxidation to docosahexanoic, arachidonic acid in the kidney were statistically significant. Treatment of rats with EM-X prior to the intraperitoneal administration of Fe-NTA led to a reduction in the overall levels of conjugated dienes (CD) measured in the kidney by 27% and in the liver by 19% suggesting inhibition of lipid peroxidation in these organs. The levels of glutathione and alpha-tocopherol were largely unaffected suggesting that the protection by the regular strength of EM-X was confined to the inhibition of lipid peroxidation in vivo, a point dependent on the concentrations of bioactive flavonoids.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12270679&dopt=Abstract



Hepatol Res. 2002 Oct;24(2):125.
Toxic effects of sunflower oil on ethanol treated rats.

Aruna K, Kalpana C, Viswanathan P, Menon V.

Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India

In the present study, we investigated the effect of raw as well as thermally oxidized sunflower oil (commercially available) on ethanol induced hepatotoxicity. Ethanol was given to animals at a level of 20% and sunflower oil at a level of 15%. Results show higher activity of plasma aspartate transaminase (AST) and alkaline phosphatase (ALP) and also higher levels of plasma and tissue cholesterol, phospholipids and triglycerides both in alcohol+raw as well as thermally oxidized oil groups. The level of cholesterol and triglycerides increased significantly in the liver of rats given alcohol alone, alcohol and raw as well as thermally oxidized oil but the level of phospholipids decreased. The activity of phospholipase A and phospholipase C in liver was found to be increased significantly in alcohol alone, alcohol+oil groups as compared to control group. Histopathological changes in the liver of alcohol and alcohol+oil groups were in good correlation with biochemical parameters. The liver samples of alcohol administered rats showed both microvesicular and macrovescicular type of fatty changes, where as alcohol+oil fed groups showed inflammatory cell infiltrate in the portal triad, microvesicular and macrovesicular type of fatty changes and feathery degeneration of hepatocytes. Studies on the phospholipid fatty acid composition in the liver showed the presence of a number of fatty acids in the alcohol and oil treated groups, which are not present in the control group. The results obtained thus indicate hepatotoxic and hyperlipidaemic effects of alcohol and oil given together.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12270741&dopt=Abstract [PubMed - as supplied by publisher]



mbd.sphere.ne.jp

OBJECTIVES: Several methods for measuring lysophosphatidylcholine (LPC) concentrations have been reported. However, these methods are not practical because they are either too complicated and/or too time-consuming for LPC determinations in human serum and plasma. DESIGN AND METHODS: We have developed a new enzymatic LPC assay, which uses lysophospholipase, glycerophosphorylcholine phosphodiesterase and choline oxidase, and which determines the quantities of hydrogen peroxide generated in the presence of peroxidase using an oxidative chromogenic reagent and 4-aminoantipyrine. RESULTS: Various samples were mixed with LPC assay reagents, and their changes in absorbance were measured. The present method produced a linear calibration line between LPC concentration and absorbance change. It also measured only LPC, and not other phospholipids such as phosphatidylcholine, sphingomyelin and lysophosphatidic acid. The within-run and between-run coefficients of variation were 0.3-0.7% and 0.7%, respectively. The recovery of exogenous LPC added to control serum was 99.5-102.1%. The correlation coefficient obtained in a comparison with a method for analyzing fatty acids was 0.9122. CONCLUSIONS: The present method is simple, specific for LPC, and can be applied with an automatic analyzer. It may also be useful for further studies of the biological functions of LPC as well as clinical applications in various disorders. 2002 The Canadian Society of Clinical Chemists


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12270773&dopt=Abstract [PubMed - in process]








Hair loss is a problem in modern soceity. Examining the factors of hair growth may shed light on how hair loss might occur. How long can hair grow before it stops growing eventually if it does? Given that the hair growth rate is quite uniform and constant, somewhere between 0.3-0.5 millimeters per day, it's believed that the length of anagen, the growth phase, differs among individuals, and this is the major determinant to the maximum hair length. For some individuals, anagen may last ten years. Of course the length of the anagen is governed by genes, and the genetic background of the individuals. Non-genetic factors such as nutritional condition, weather, seasonal changes (hair may grow a bit faster during winter), taking medications, health condition may of course influence the rate of hair growth as well as hair loss. The shape of the hair, straight or curly, is dependent on the shape of the follicle. A circular or round hair follicle would generate straight hair, while the follicle with oval or elliptical shapes (in its cross-section) would produce a curly hair.














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