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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5 || Follicle and follicular cells research abs 1

Zuchthygiene. 1970;5(1):11-26.
[Prophylaxis of hormonal sterility in the cow: II. Observations during and after the use of gestagen postpartum]

[Article in German]

Schmidt H, Huber E, Gunzler O, Jochle W, Smidt D.

PIP: 183 Holstein cows were studied during and after administration of ch lormadinone acetate (CAP) postpartum. CAP was either fed orally (10mg/d ay for 15 or 20 days) or applied parenterally twice (50 mg) or once (100 mg). By oral administration, estrus was completely (=100%) and ovulation mostly (greater than 95%) suppressed, but follicle growth and estrogen production in growing follicles was not inhibited. Out of 39 closely observed animals, 89.7% ovulated after CAP withdrawal, 79.5% of them within 96 hours. With all animals inseminated, 37.5% conceived at this 1st ovulation. In 144 cows, of which 101 were orally treated with CAP and 39 by injection, shortening of the calving interval and a reduction in fertility problems was achieved. A partly significant improvement of the overall conception rate, the curtailing of the interval between parturition and conception as well as a marked reduction of the standard deviations from the mean values of those intervals were observed. Parenterally treated cows responded in a similar way. But since their 1st ovulations were spread over several weeks and since at these 1st ovulations silent heat was a considerable problem, no prediction of the timing of the 1st ovulation could be made. On the basis of the data presented on uterine contractility, ovarian functions, vestibular hyperaemia, and mucus secretion during and after CAP administration are discussed.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12304677&dopt=Abstract

Res Steroids (Amst). 1966;2:389-92.
Probable mode of action of oral contraceptives.

Diczfalusy E.

PIP: It has been shown that the gestagen component of oral contraceptives changes the composition of the cervical mucus so as to make sperm penetration difficult. Endometrial changes and changes in the normal patterns of tubal and uterine motility have been described. Ovulation is inhibited by inhibition of ovarian response to gonadotrophins or by inhibition of the formation and/or release of endogenous gonadotrophins. The "classical" pill inhibits ovulation by interfereing with the formation or release of luteinizing hormone (LH). The sequential form of oral contraception has a relatively high dose of estrogen which inhibits secretion of both LH and follicle stimulating hormone (FSH); the gestagen component causes better shedding of the endometrium. When very small doses of gestagens are given continuously, ovulation is usually not inhibited. Biochemical rather than morphological endometrial change may be most important. It is concluded that the mode of action of oral contraceptives is not completely understood. Discussion by others follows the paper.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12305024&dopt=Abstract

West J Surg Obstet Gynecol. 1964 July-August;72:222-9.
Control of fertility with oral medication.

Diddle AW, Watts GF, Gardner WH, Williamson PJ.

PIP: 447 women were treated with either norethindrone, norethindrone acetate or norethindrone with ethinyl estradiol to prevent conception. The total length of time patients remained under treatment varied from 1 to 60 months for an over-all total experience of 8308 cycles. Only 1 woman conceived, and she had not followed instructions. 4 others were pregnant when treatment was instituted, as learned by hindsight. 58 women conceived within 1 to 12 months after they discontinued medication taken previously for periods of time ranging from 1 to 31 months. Most conceived within 3 months. None of the women suffered thrombophelbitis. Breakthrough uterine bleeding was more common with the administration of norethindrone or norethindrone acetate than with norethindrone acetate combined with ethinyl estradiol. Nausea, retention of fluid, and mastalgia were more common with the progestin-estrogen combination. Ophthalmologic examinations, 17-ketosteroid studies, follicle-stimulating hormone analysis, and blood cholesterol determinations on patients selected at random and after varying periods of treatment were within normal limits.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12305556&dopt=Abstract

J Res Indian Med. 1970;4(2):143-51.
Antifertility effect of betal leaf stalk (Tambul patrabrint): a preliminary experimental study.

Tewari PV, Chaturvedi C, Dixit SN.

PIP: This study was undertaken to determine possible antifertility properties of the stalk of the betel plant, Piper betle, which is extensively cultivated in warm moist parts of India for its leaves. The thin stalks were dried, powdered, and extracted with alcohol; the residue was used in the study and named betel leaf stalk extract (BLSE). It was macerated with gum acacia, suspended in distilled water, and diluted to 25 mg/ml. The drug was fed by oral intubation. Initially 20 rats were used in 2 groups, one receiving BLSE 50 mg/kg body weight for 10 days and the other vehicle only. Both were caged with males for 15 days. Only 2 of 10 rats receiving BLSE gave birth to litters while 9 of 10 controls delivered. Number of pups was also reduced. In another experiment using 60 female rats to 30 while BLSE at 50 mg/100 gm body weight was fed for 3 days during diestrus to 30 while 30 controls received vehicle only. Successful mating was confirmed by sperm in vaginal smears. 8 of 30 treated rats delivered 3.5 pups each while 29 of 30 controls delivered 7 pups per rat. In a third experiment 10 female rats were given BLSE 50mg/100 gm body weight, 10 vehicle only, and 10 neoclinesterol 150 mcg/100 gm body weight for 4 consecutive days. On Day 5 the animals were sacrificed and weights of uterus and ovaries recorded. Antiestrogenic properties were studied in oophorectomized rats given stilbestrol 150 mcg/100 gm body weight by injection, then fed 150 mcg/100 gm body weight of neoclinesterol or BLSE, 50 mg/100 gm, but the neoclinesterol. Vaginal smears were examined for cornification on Days 2, 3, and 4. Progestational activity was studied in 12 immature female rabbits injected with stilbestrol then treated with BLSE or leutocycline. Tests for estrogenic and antiestrogenic activity of BLSE were negative. A mild progestational activity was found in immature estrogen-primed rabbits but some follicle depressant type action was noted as several graffian follicles were seen in their regressive phase when ovaries were sectioned. Similar findings were obtained in rats treated after spaying. Results show BLSE has a definite antifertility activity. When fed in diestrus phase the estrus cycle was not altered nor was any estrogenic or antiestrogenic effect noted.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12305561&dopt=Abstract

Pak J Sci Ind Res. 1971 February-April;14(1-2):118-20.
Some chemical modifications of human follicle stimulating hormone (FSH).

Amir SM, Jenkins JF.

PIP: Details are presented of a study of the effects of a range of chemical reactions on the biological activity of pituitary FSH. The chemical modifications included acetylation, succinylation, guanidination, alkylation, and esterification. Both the acetylation and the succinylation reactions caused complete loss of FSH biological activity which could not be restored by de-O-acetylation with hydroxylamine. More than 50% of the activity was retained after guanidination with O-methylisourea. Carbamylation by the use of K carbon-14 NO caused no loss of activity. Alkylation either with iodoacetamide or acrylonitrile did not effect the activity, 90% of which was retained when disulphide groups were reduced and this followed by alkylation. The use of 8 M urea to alter the tertiary structure of the glycoprotein, followed by cleavage and alkylation of disulphide linkages, resulted in estensive loss of activity. Treatment with 8 M urea alone under similar conditions diminished the activity by 35%. Almost complete loss of activity was also observed on esterification of FSH. It is suggested that the last effect may have been caused by the modification of N-acetyl neuraminic acid residues, known to be essential for the complete manifestation of the biological activity. The authors complete manifestation of the biological activity . The authors suggest that, as the chemical reagents used for the modification of glycoproteins frequently suffer from the disadvantages of being non-specific, further chemical and conformational characterization will be required before the exact nature of the structural changes taking place in FSH molecule and their effect on biological activity can be fully established.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12305951&dopt=Abstract

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