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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5

Klin Lab Diagn. 2002 Jul;(7):9-11.
[Salivary thyroid hormones in evaluation of the functional state of the hypophyseal-thyroid system]

[Article in Russian]

Gotovtseva LP, Korot'ko GF.

Thyrotropic hormone (TTH), total thyroxin (TT4), total triiodothyronine (TT3), free thyroxin (FT4), and free triiodothyroxine (FT3) were measured in the serum and oral liquid of clinically healthy subjects and patients with hypo- and hyperthyrosis. A relationship between hormone concentrations in the serum and oral liquid and the function of the pituitary-thyroid system was revealed. FT4, FT3, and TTH concentrations in the serum and oral fluid were in high correlation. This is regarded as an evidence of possible recretory origin of these hormones in the saliva.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12187594&dopt=Abstract

J Sci Med Sport. 2002 Jun;5(2):108-14.
Relationship between serum, saliva and urinary cortisol and its implication during recovery from training.

Neary JP, Malbon L, McKenzie DC.

Faculty of Kinesiology, University of New Brunswick, Fredericton, Canada.

This study examined the relationship among resting cortisol levels measured in serum (SER), saliva (SAL). overnight urine (ON) and 24 h urinary free cortisol (24 h) samples to determine which would be most appropriate for monitoring recovery from the physiological stress imposed by exercise training. Eight (3 female, 5 male) physical education students (X+/-SD age= 22+/-2 y, mass= 73.6+/-17.1 kg; predicted 120m shuttle] VO2max = 3.83+/-1.34 L x min(-1); 51.4+/-8.0 mL x kg(-l) x min(-1)) volunteered. Venous blood, saliva and urine were collected following one day of recovery from training. Morning (0730-0800 h) cortisol was analysed commercially using solid phase radioimmunoassay. Mean resting cortisol concentrations for these samples were in the high end of the normal reference interval previously reported for this population in the literature: SER (656.5+/-122.3 nmol x L(-1)). SAL (41.9+/-12.3 nmol x L(-1)), ON (72.0+/-19.5 nmol x L(-1)) and 24 h (170.8 +/-58.7 nmol x day(-1)). Intraclass correlation coefficients (R) showed a high correlation between 24 h versus SER (R=0.99), SAL (R=0.97), and ON (R=0.97) cortisol, and between SER and SAL cortisol (r=0.99) Limits of agreement analysis to determine measurement error also revealed significant correlations (r2=0.60-0.99) between the dependent variables. In conclusion, the strong intraclass correlation coefficient between 24 h cortisol versus SAL and SER suggests that either sample can be used to monitor cortisol hormone during a recovery period from exercise training. The use of SAL samples for cortisol analysis may have many advantages due to its non-invasive nature.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188082&dopt=Abstract

J Med Assoc Thai. 2002 May;85(5):583-9.
Risk of breast cancer in post-menopausal women using hormone replacement therapy.

Ratanawichitrasin A, Reansuwan W, Ratanawichitrasin S, Bhodhisuwan K, Kongpatanakul S.

Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

OBJECTIVE: To study the relationship of hormone replacement therapy (HRT) in post-menopausal women and risk of breast cancer. PATIENTS AND METHOD: The authors conducted a case-control study comparing the proportion of HRT used between breast cancer and non-breast-cancer women. Cases were breast cancer patients who had natural menopause (excluded hysterectomy) and aged > or = 50-years-old from the Siriraj Breast Cancer database (1983-1996). Controls were post-menopausal volunteers aged 50 year or older who visited Siriraj Hospital for other purposes such as elderly clinics, health check, etc. After informed consent, well-trained surgeons examined the women in the control group to exclude any potential breast cancer. Patient characteristics and risk factors were collected. RESULTS: Of 1,913 patients in the database, 623 were included as the cases. Data from 679 volunteers were collected for controls from May to December 1999. Among 1,302 of the study population 58 women had ever used HRT (4.5%), which distributed to 3.2 per cent (20/623) in cases and 5.6 per cent (38/679) in controls. From univariate analysis, age, age at menopause, number of children, habitat, education, contraceptive pills, familial history of breast cancer and HRT usage were associated with breast cancer (p-value<0.05). After multivariate forward stepwise logistic regression analysis, there was no association between HRT use and breast cancer (adjusted odds ratio (OR) = 0.61, 95% CI = 0.31-1.20). In subgroups analysis, women who had older age, higher education level, history of taking contraceptive pills, or positive familial history of breast cancer in second degree relatives had a decreased risk of breast cancer, while those living outside Bangkok had an increased risk. CONCLUSION: Hormonal replacement therapy in post-menopausal women was not associated with increased risk of breast cancer.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188389&dopt=Abstract

J Am Chem Soc. 2002 Aug 28;124(34):10236-47.
Conformational preferences of jet-cooled melatonin: probing trans- and cis-amide regions of the potential energy surface.

Florio GM, Christie RA, Jordan KD, Zwier TS.

Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393, USA.

The hormone melatonin (N-acetyl-5-methoxytryptamine) is an indole derivative with a flexible peptide-like side chain attached at the C3 position. Using a combination of two-color resonant two-photon ionization (2C-R2PI), laser-induced fluorescence excitation (LIF), resonant ion-dip infrared spectroscopy (RIDIRS), fluorescence-dip infrared spectroscopy (FDIRS), and UV-UV hole-burning spectroscopy, the conformational preferences of melatonin in a molecular beam have been determined. Three major trans-amide conformers and two minor cis-amide conformers have been identified in the R2PI spectrum and characterized with RIDIRS and FDIRS. Structural assignments are made using the infrared spectra in concert with density functional theory and localized MP2 calculations. Observation of cis-amide melatonin conformers in the molecular beam, despite the large energy gap (approximately 3 kcal/mol) between trans- and cis-amides, is striking because there are at least nine lower-energy trans-amide minima that are not detected. The implications of this observation for cooling and trapping conformational population in a supersonic expansion are discussed.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188688&dopt=Abstract

BMC Physiol. 2002 Aug 21;2(1):14.
Reduced natriuretic response to acute sodium loading in COMT Gene deleted mice.

Odlind C, Reenila I I, Mannisto PT, Juvonen R, Uhlen S, Gogos JA, Karayiorgou M, Hansell P.

Dept of Medical Cell Biology, University of Uppsala, Biomedical Centre, Uppsala, Sweden. peter.hanselhysiology.uu.se

BACKGROUND: The intrarenal natriuretic hormone dopamine (DA) is metabolised by catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO). Inhibition of COMT, as opposed to MAO, results in a potent natriuretic response in the rat. The present study in anaesthetized homozygous and heterozygous COMT gene deleted mice attempted to further elucidate the importance of COMT in renal DA and sodium handling. After acute intravenous isotonic sodium loading, renal function was followed. RESULTS: COMT activity in heterozygous mice was about half of that in wild type mice and was zero in the homozygous mice. MAO activity did not differ between the genotypes. Urinary sodium excretion increased 10-fold after sodium loading in wild type mice. In heterozygous and homozygous mice, the natriuretic effects of sodium loading were only 29 % and 39 %, respectively, of that in wild type mice. Arterial pressure and glomerular filtration rate did not differ between genotypes. Baseline norepinephrine and DA excretions in urine were elevated in the homozygous, but not in heterozygous, COMT gene deleted mice. Urinary DA excretion increased after isotonic sodium loading in the wild type mice but not in the COMT gene deleted mice. CONCLUSIONS: Mice with reduced or absent COMT activity have altered metabolism of catecholamines and are unable to increase renal DA activity and produce normal natriuresis in response to acute sodium loading. The results support the hypothesis that COMT has an important role in the DA-mediated regulation of renal sodium excretion.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188925&dopt=Abstract [PubMed - as supplied by publisher]

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