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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5

J Reprod Fertil Suppl. 1979;(26):135-46.
Development of a radioimmunoassay for human seminal plasma inhibin.

Vaze AY, Thakur AN, Sheth AR.

Using a homogeneous inhibin preparation from human seminal plasma with a molecular weight of about 19 000, a sensitive and specific radioimmunoassay (RIA) for inhibin has been developed. None of the purified hormones tested, such as LH, FSH and prolactin from different species, showed any cross-reaction in this RIA. Steroid hormones such as testosterone, dihydrotestosterone, oestradiol-17 beta and progesterone did not interfere with the assay. The antiserum had an affinity constant (Ka) of 2.379 X 10(9). The assay sensitivity was 10-15 ng per tube and the intra- and inter-assay coefficients of variation were 5-7% (n = 6) and 15% (n = 10) respectively. The recovery for inhibin added to the serum of a castrated man was 95-110%. Using this RIA, inhibin levels in various biological fluids and tissues were measured. Normo-spermic semen contained significantly higher levels of inhibin than did oligospermic semen. Human prostate contained a substantial quantity of inhibin. Monkey semen, rat serum, and bovine, ovine and porcine follicular fluids cross-reacted in the RIA, while ram testicular inhibin and bull semen did not do so. In developing (9-28 days of age) male rats, circulating inhibin levels showed an inverse relationship with serum FSH levels. In female rats of this age endogenous inhibin concentrations changed in parallel with those of serum FSH.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=118250&dopt=Abstract

Neoplasma. 1979;26(4):477-82.
Hormone dependency of rat mammary carcinoma--a comparison of two assay systems.

Sarkar S, Seshadri R, Shah PN, Kothari LS, Ghosh SN.

Succinic dehydrogenase activity of the DMBA induced tumor explants cultured with or without hormones was assessed histochemically while receptors for estrogen (ER) and progesterone (PgR) were estimated from the cytosol fraction of the tumor tissue. Tumor regression following ovariectomy (OVX) was kept as the end point for determining hormone dependency. By in vitro method positive correlation was observed in 5 of 6 responsive or hormone dependent tumors, and 13 out of 14 independent tumors. Presence of receptors (ER + PgR, PgR) correlated with responsiveness in 4 of 6 tumors while their absence in the non-responsive group correlated in 6 of 14 tumors. Prolactin responsive tumors did not regress following OVX even if ER + PgR or ER/PgR were present. Using the same tumor tissue the results of hormone dependency by the two methods were identical in only 9 of 20 tumors.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=118387&dopt=Abstract

Neuroendocrinology. 1979;29(6):413-7.
Thyroid-stimulating hormone and prolactin responses to thyrotropin-releasing hormone during infusion of epinephrine and propranolol in man.

Rogol AD, Reeves GD, Varma MM, Blizzard RM.

Male volunteers were administered 100 microgram thyrotropin-releasing hormone (TRH) intravenously during control (saline) and drug (epinephrine-propranolol) infusions. There were no differences in the thyroid-stimulating hormone (TSH) or prolactin responses to TRH during the epinephrine-propranolol infusion periods. There were no significant differences in growth hormone (GH) responses to epinephrine-propranolol infusions. Epinephrine-propranolol had no detectable effect on basal TSH, prolactin and GH concentrations. We conclude that the alpha-adrenergic system does not play any role at the pituitary level in modulating the effect of TRH-stimulated TSH or prolactin secretion in male volunteers.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=118391&dopt=Abstract

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5905-9.
Biochemical and morphologic studies on diabetic rats: effects of sucrose-enriched diet in rats with pancreatic islet transplants.

Eder HA, Novikoff PM, Novikoff AB, Yam A, Beyer M, Gidez LI.

Isolated pancreatic islets were administered to Lewis rats with streptozotocin-induced diabetes. Then the rats were fed either a semisynthetic diet containing 60% (wt/wt) sucrose for 3 weeks or were continued on chow. Transplantation resulted in a decrease in serum glucose, an increase in serum insulin, and a marked decrease in serum triacylglycerol, particularly in the sucrose-fed diabetic rats. In these rats, in demarcated areas of hepatocytes surrounding portal vein termini, lipid was deposited in the cytosol and large lipoprotein particles engorged the Golgi apparatus, Golgi-derived secretory vacuoles, and GERL. This model permits observation of the effects of pancreatic islet hormones on lipogenesis by hepatocytes in situ.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=118459&dopt=Abstract

Proc Natl Acad Sci U S A. 1979 Nov;76(11):6017-20.
Membrane potential changes caused by thyrotropin-releasing hormone in the clonal GH3 cell and their relationship to secretion of pituitary hormone.

Ozawa S, Kimura N.

Effects of thyrotropin-releasing hormone (TRH; thyroliberin) on membrane electrical properties were studied in the clonal rat anterior pituitary cell (GH3) by continuous recording of the intracellular potential. Application of TRH, which stimulates the release of prolactin and growth hormone from the GH3 cell, elicited a transient hyperpolarization of the cell membrane followed by an enhancement of the generation of action potentials for an extended period in the majority of cells tested. The transient hyperpolarization was due to an increase of the membrane conductance to K+. The enhancement of the spike generation was not due to membrane depolarization. The input resistance of the cell membrane was found to be increased during the facilitation. Thus the mechanism of the facilitatory action of TRH is different from the mechanisms of conventional excitatory neurotransmitters. TRH enhances the spike generation, thus promoting Ca2+ entry from extra- to intracellular space (the action potential of the GH3 cell has a Ca2+ component) and stimulating the release of hormones. This notion is supported by the observation that cobalt ions, which block the calcium spike in these cells, completely abolished the stimulatory effect of TRH on the release of prolactin and growth hormone.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=118460&dopt=Abstract

Loss of hair changes the appearance of a person, and the identity of the person in social context to a certain extent. Hair growth is a complex biological process, which has not yet been completely understood. A multitude of therapeutic measures, including drugs, surgery, and suppelements have been made available, and used. However, due to the diversity of the problems underlying hair loss, there is no single solution for all hair loss cases. Most of chemical drugs and hair transplantation surgeries are not free from varying degrees of undesirable side effects on health.

Hair Million is an alternative solution to hair loss problems. Albeit only anecdotally, it has demonstrated efficacy in the improvement for age-related hair thinning and hair loss for a significant fraction of people who take it as recommended. We do not know the mechanisms of action as to how Hair Million works to help stop hair loss, and promote hair growth. We only know by anecdotal observations. There has been no clinical trials nor placebo controlled statistical analysis.

DHEA is a natural hormone, and it is produced in our body by the adrenal glands. DHEA has been suggested to provide numerous potential benefits. DHEA (or dehydroepiandrosterone) is converted into androgens (male hormones) or estrogens (female hormones) in the cells.

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