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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5

Eur J Neurosci. 2002 Nov;16(9):1751-60.
Overexpression of corticotropin-releasing hormone in transgenic mice and chronic stress-like autonomic and physiological alterations.

Dirks A, Groenink L, Bouwknecht JA, Hijzen TH, Van Der Gugten J, Ronken E, Verbeek JS, Veening JG, Dederen PJ, Korosi A, Schoolderman LF, Roubos EW, Olivier B.

Department of Psychopharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. A.Dirkharm.uu.nl

To gain a greater insight into the relationship between hyperactivity of the corticotropin-releasing hormone (CRH) system and autonomic and physiological changes associated with chronic stress, we developed a transgenic mouse model of central CRH overproduction. The extent of central and peripheral CRH overexpression, and the amount of bioactive CRH in the hypothalamus were determined in two lines of CRH-overexpressing (CRH-OE) mice. Furthermore, 24 h patterns of body temperature, heart rate, and activity were assessed using radiotelemetry, as well as cumulative water and food consumption and body weight gain over a 7-day period. CRH-OE mice showed increased amounts of CRH peptide and mRNA only in the central nervous system. Despite the presence of the same CRH transgene in their genome, only in one of the two established lines of CRH-OE mice (line 2122, but not 2123) was overexpression of CRH associated with increased levels of bioactive CRH in the hypothalamus, increased body temperature and heart rate (predominantly during the light (inactive) phase of the diurnal cycle), decreased heart rate variability during the dark (active) phase, and increased food and water consumption, when compared with littermate wildtype mice. Because line 2122 of the CRH transgenic mice showed chronic stress-like neuroendocrine and autonomic changes, these mice appear to represent a valid animal model for chronic stress and might be valuable in the research on the consequences of CRH excess in situations of chronic stress.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12431228&dopt=Abstract

Rev Rhum Mal Osteoartic. 1976 Feb;43(2):105-11.
[Biological exploration of Paget's disease]

[Article in French]

Eisinger J, Laponche AM, Roux H, Recordier AM.

The laboratory investigation of cases of Paget's disease has revealed results of three types :--small increases in the sedimentation rate, in the ceruloplasmin level, in the average corpuscule volume, and in uricaemia. These changes are of little practical value;--a considerable increase in the common stocks and in the turn-over of calcium, a frequently negative assessment with a parathyroid hormone level at the lower limit of normality. The levels of calcaemia and calciuria, little changed on average, may increase slightly after hospitalization. The use of these parameters is valuable but they are sometimes difficult to interpret;--an increase in the levels of alkaline phosphatases and of urinary hydroxyproline, in relation to the extent and the activity of the disease, and also of certain parameters of calcium-45 and of quantitative histology. A statistical study of the spontaneous evolution of hydroxyprolinuria in 50 patients with Paget's disease who were not treated for less than 3 years, allowed the authors to establish in what conditions a change induced by calcitonin or mithramycin is significant.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=131366&dopt=Abstract

Cancer Res. 1976 Jun;36(6):1883-5.
Plasma hormone profiles in populations at different risk for breast cancer.

Hill P, Wynder EL, Helman P, Hickman R, Rona G.

Evidence suggests that the reliability of urinary androgens or estrogens as prognostic discriminants for breast cancer may vary with different populations and may bear little relation to changes in plasma hormone levels. In this study the plasma estradiol level was significantly lower in Bantu, but did not in Caucasian, women with breast cancer, while the proportion of plasma androstenedione to dehydroepiandrosterone increased in Bantu patients. These changes in the plasma hormone profile in Bantu patients, a low-risk population for breast cancer, imply a change in ovarian and/or adrenal activity. Lack of similar hormone changes in Caucasian patients may be due to the initial differences in the hormone profile between the healthy Caucasian and Bantu women and to the different environmental factors that promote the breast disease in Western women.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=131645&dopt=Abstract

Circ Res. 1976 May;38(5 Suppl 1):I22-30.
Effects of mechanical activity and hormones on myocardial glucose and fatty acid utilization.

Neely JR, Whitmer M, Mochizuki S.

The effects of increased cardiac work on glycolysis, the citric acid cycle, and oxidation of fatty acids were studied in isolated rat hearts. Glycolysis was stimulated by increased work in heart perfused with glucose alone or with glucose, high levels of insulin, and low levels of palmitate. With glucose alone, stimulation was associated with a rapid decrease in phosphate potential and rapid activation of phosphofructokinase, but an apparently slower activation of glucose transport. With glucose, insulin, and palmitate present, stimulation of glucose utilization was rapid and correlated with activation of phosphofructokinase.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=131653&dopt=Abstract

Diabetologia. 1976 May;12(2):137-43.
Human adipose tissue in culture V. Studies on the metabolic effects of insulin.

Smith U, Bostrom S, Johansson R, Nyberg G.

Specimens of human adipose tissue were cultured for one week with or without the addition of insulin. The basal as well as the noradenaline-stimulated lipolysis were enhanced in the explants cultured with insulin, showing that the long-term effect of the hormone is lipolytic. However, an acute antilipolytic effect of insulin could be demonstrated in these explants in the subsequent short-term incubations. The basal rate of glucose incorporation into the lipids was enhanced in the explants cultured with insulin. When insulin was added in the short-term incubations these explants did not further respond to the hormone while this was the case with the explants cultured without insulin. Thus, it seems that prolonged exposure to insulin leads to a diminished acute effect of the hormone on glucose metabolism. However, the same explants responded to the antilipolytic effect showing that insulin was able to bind itself to the membrane. The activities of hexokinase (HK), glucose-6-phosphage dehydrogenase (G6PDH), pyruvate kinase (PK) and lactate dehydrogenase (LDH) were increased in large fat cells both in freshly excised tissue and in cultured explants. However, the activity of phosphofructokinase (PFK) did not correlate with the cell size. The presence of insulin during the culture period enhanced the activities of G7PDH, PK, and LDH, while this was not found for HK or PFK. The data thus suggest that the metabolic capacity of human fat cells is enhanced by long-term exposure to insulin. Although enzyme induction could be shown for G6PDH, PK and LDH it seems unlikely that this is of importance for the increased rates of glucose metabolism in these explants since the rate-limiting enzymes, HK and PGK, were not increased. Most probably, then, this stimulating effect of insulin is exerted on the membrane and the rate of glucose transport.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=131727&dopt=Abstract

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