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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5

Izv Akad Nauk Ser Biol. 2002 Jan-Feb;(1):55-62.
[Influence of phytohormones on development of conidial inoculum of causative agents of the phlox and barley powdery mildew]

[Article in Russian]

Mishina GN, Talieva MN, Babosha AV, Serezhkina GV, Andreev LN.

Tsitsin Main Botanical Gardens, Russian Academy of Sciences, ul. Botanicheskaya 4, Moscow, 127276 Russia.

We studied the role of phytohormones: zeatin, kinetin, and abscisic acid, in the regulation of development of the conidial inoculum of Erysiphe cichoracearum DC. f. phlogis Jacz. and E. graminis DC. f. hordei Marchal. When the pathogen conidia were in direct contact with phytohormones, the intensity of their germination significantly increased. In the presence of cytokinins, the amount of normal appressoria decreased and that of abnormal growth tubes increased. On the phlox leaves treated with cytokinins, the intensity of germination of the conidia increased, as compared to the control, while abscisic acid exerted the opposite effect. The treatment of barley leaves with cytokinins did not affect markedly the development of conidial inoculum, as compared to the control, while abscisic acid significantly decreased the intensity of germination of the conidia. On the leaves of different Phlox species, the degree of germination of the conidial negative correlated with their resistance against the powdery mildew. The role of cytokinins in pathogenesis of biotrophic fungi is discussed.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12068440&dopt=Abstract

Vopr Med Khim. 2002 Jan-Feb;48(1):121-30.
[Screening of mutations in genes of pro-opiomelanocortin in patients with constitutional exogenous obesity]

[Article in Russian]

Pankov IuA, Iatsyshina SB, Karpova SK, Chekhranova MK, Popova IuP, Grigorian ON, Rogaev EI.

Endocrine Research Centre of RAMN, 115478, Moscow, Moscyorechye str, 1, Russian Federation. yuri-pankotu-net.ru

Proopiomelanocortin (POMC) is a precursor of ACTH, beta- and gamma-liportopins, alpha-, beta- and gamma-MSH, beta-endorphin. alpha-, beta- and gamma-MSH are synthesized by hypothalamus neurons, and leptin stimulates their synthesis. These hormones regulate food consumption and energy metabolism by via melanocortin receptors (MC3-R and MC4-R) in hypothalamus. Screening mutations in the coding region of human POMC has been carried out with PCR, SSCP and DNA sequencing and the association study of these mutations and human obesity has been performed. Group of patients with the exogenous obesity (BMI 37.8 +/- 6.8 kg/m2) consisted of 228 persons (173 women and 55 men). 145 blood donors (67 women and 78 men) without obesity (BMI J25 kg/m2, 23.1 +/- 2.2 kg/m2) and 170 women without apparent obesity at the beginning of the study were included in the control group. 8 polymorph sites: insertions; missense and silent mutations have been identified in the coding region of POMC. Among them 1) two heterozygous mutations: the insertion of 6 b.p. (GGGCCC) in codon 176 inducing the insertion of two amino acid residues (Arg-Ala) in POMC and nonsense mutation (G-7316-T) in codon 180 of gamma-LTH coding region of the same DNA chain were identified in 4 women (5.8%) out of 69 patients with morbid obesity (BMI 40-53 kg/m2). These mutations were not found in control (n = 315). 2) The new heterozygous mutation T-7130-C (Phe118Leu) in active site of alpha-MSH has been identified in POMC gene of a woman suffering with obesity since the early childhood. 3) Mutation A-7341-G (Glu188Gly) seemed to have a protective effect because it was revealed more frequently in control (3.9%) than in obese patients (0.66%). The results of genetic study of two pedigrees suggested the dominant influence of the first two mutations (1 and 2) on woman obesity.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12068494&dopt=Abstract

Arq Gastroenterol. 2001 Oct-Dec;38(4):254-60.
[Thyroid function tests in viral chronic hepatitis]

[Article in Portuguese]

Novis M, Vaisman M, Coelho HS.

Servico de Gastroenterologia, Hospital Universitario Clementino Fraga Filho, Departamento de Clinica Medica, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, FM-UFRJ.

BACKGROUND: One hundred and twenty five patients with virus B or C chronic active hepatitis and postnecrotic cirrhosis and different degrees of liver dysfunction were studied. AIM: 1) To determine a thyroid hormonal profile; 2) to evaluate the prognostic value of these tests in relation to the progression of the disease and mortality; 3) compare these findings with Child-Pugh classification. PATIENTS AND METHODS: The patients were divided in four groups: a) 31 with chronic active hepatitis; b) 41 with postnecrotic cirrhosis Child A; c) 35 with postnecrotic cirrhosis Child B and d) 18 with postnecrotic cirrhosis Child C. The protocol comprised serum measurements of albumin and bilirrubin, estimates of prothrombin time and clinical evaluation of ascites and encephalopathy, measurement of total serum triiodothyronine, thyroxine, thyroid-stimulating hormone, free thyroxine, reverse triiosothyronine, calculated rT3/T3 index (IrT3) and thyrotropin-releasing hormone test. RESULTS: Total serum triiodothyromnine showed the most significant difference among the groups, gradually lower as the disease became more advanced (CAH: 149.2 +/- 42.3 ng/dL; PNC-A: 137.4 +/- 37.2 ng/dL; PNC-B: 88.0 +/- 28.4 ng/dL and PNC-C: 41.8 +/- 21.9 ng/dL). Low levels of T4 (4.5 +/- 2.0 micrograms/dL) and FT4 (0.7 +/- 0.4 ng/dL) and elevated levels of thyroid-stimulating hormone (7.2 +/- 11.5 microIU/mL), reverse triiosothyronine (60.8 +/- 52.1 ng/dL) and calculated rT3/T3 index (2.2 +/- 2.6) were more frequent in patients with postnecrotic cirrhosis Child C. Thyrotropin-releasing hormone test was normal in the majority of the patients. CONCLUSION: The present study shows a positive relationship between the low serum levels of T3 and elevated serum levels of rT3 and IrT3/T3 with the degree of hepatic dysfunction according to the Child-Pugh classification.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12068536&dopt=Abstract

Genetika. 2002 May;38(5):629-34.
[The detection of genome polymorphism in Stachys species using RAPD]

[Article in Russian]

Kochieva EZ, Khussein IA, Legkobit MP, Khadeeva NV.

Vavilov Institute of General Genetics, Russian Academy of Sciences, Timiryazev Agricultural Academy, Moscow, Russia. kochievigg.ru

Molecular genome analysis was for the first time carried out in the genus Stachys. RAPD analysis proved to be suitable for identifying the species-specific markers, studying the interspecific DNA polymorphism, and detecting the genetic changes that arise during in vitro culturing of Stachys sieboldii. In addition, RAPD was used for screening genetic variation in S. sieboldii regenerants obtained at various phytohormone concentrations. High cytokinin concentrations and multiple regeneration were shown to induce genetic changes detectable with RAPD patterns. High DNA polymorphism was demonstrated for two types of S. sieboldii callus cultures and for plants regenerated from a callus culture.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12068546&dopt=Abstract

Ontogenez. 2002 May-Jun;33(3):170-5.
[Production of regenerants in sugar beet]

[Article in Russian]

Kolodiazhnaia IaS, Deineko EV.

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 10, Novosibirsk, 630090 Russia.

The experimental results are presented on production of plants-regenerants of the sugar beet (Beta vulgaris L.) via callus formation or direct organogenesis from the leaf tissues. The method of aseptic treatment was developed for the seeds with strong bacterial and fungal invasion. The regenerant plants were obtained in the presence of various concentrations of synthetic hormones, such as cytokinin (6-benzaminopurine) and auxin (naphthylacetic acid), and inhibitor of auxin transport in plants (2,3,5-triiodobenzoic acid). This combination of growth regulators made it possible to avoid callus formation. The genotype of initial plants affected the capacity for callus formation and regeneration. The temperature influenced rhizogenesis in regenerants.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12068716&dopt=Abstract

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