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Pathogen research abs 1 || Pathogen research abs 2 || Pathogen research abs 3 || Pathogen research abs 4 || Pathogen research abs 5 || Hormone and endocrine research abs 1 || Hormone and endocrine research abs 2 || Hormone and endocrine research abs 3 || Hormone and endocrine research abs 4 || Hormone and endocrine research abs 5

Environ Monit Assess. 2002 Oct;79(2):193-215.
Assessment of contamination and biomarker responses in two species of herons on the St. Lawrence river.

Champoux L, Rodrigue J, Desgranges JL, Trudeau S, Hontela A, Boily M, Spear P.

Environment Canada, Canadian Wildlife Service, Ste-Foy, Quebec. louise.champouc.gc.ca

This study was undertaken to validate potential biomarkers of exposure and effects due to chemical contaminants in breeding colonies of the Great Blue Heron and the Black-crowned Night-Heron on the St. Lawrence River. Eggs and fledglings from both species were collected from many colonies along the River. The fledglings from colonies in freshwater and brackish water were more contaminated by mercury and PCBs than those from estuarine and gulf colonies. With respect to fledglings of the two heron species, some morphometric and blood biochemical measurements, including plasma thyroid hormones and retinol, were significantly different among colonies. Significant differences were also observed in liver retinoids, EROD and porphyrins among colonies. The results of this study suggest that plasma retinoids and thyroid hormones are good biomarkers of exposure and effects, and are sufficiently sensitive to reflect local and regional variations in contamination. Along with the measure of contaminants in egg and plasma, they constitute non-invasive biomarkers which represent an important criteria for long term monitoring of wildlife species. It is concluded that the Great Blue Heron is an appropriate sentinel species in the surveillance network for the St. Lawrence River.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12413303&dopt=Abstract

J Trace Elem Med Biol. 2002;16(4):227-30.
Trace elements in viral hepatitis.

Kalkan A, Bulut V, Avci S, Celik I, Bingol NK.

Clinical Microbiology and Infectious Diseases Department, Firat Medical Center, Firat University, 23118, Elazig-Turkey.

In this study, serum trace elements, including selenium (Se), zinc (Zn), copper (Cu), were determined by using Atomic Absorption Spectrophotometer (SpectrAA 250 Plus Zeeman, Varian, Australia) in sera of patients with viral hepatitis (A, B, C, D, E) cases (n = 102), and statistically compared with the controls (n = 52). In viral hepatitis, Cu levels were found as 3.23 +/- 1.02 mg/L, and this value was significantly higher than the control group (1.13 +/- 0.21) (p < 0.01). Both, Se and Zn levels found to be significantly low in viral hepatitis cases (p < 0.01). While Se level was 81.4 +/- 26.01 microg/L in viral hepatitis (n = 101), it was found to be 166.15 +/- 4.58 microg/L in healthy individuals. Meanwhile, Zn levels were 0.230 +/- 0.081 mg/L and 0.748 +/- 0.392 mg/L in hepatitis cases (n = 101) and the control group, respectively. There was no difference amongst viral hepatitis groups classified in regard with agents and clinical manifestation, such as A, acute hepatitis B, chronic hepatitis B, C, D and E. Previously, it was indicated that absorption disorders in gastrointestinal system, especially in chronic cases, were not main causes of decrease of trace elements by iron and several other parameters in sera of the cases. Therefore, we suggest that decrease in Zn and Se levels and elevation in Cu levels are probably resulted from defence strategies of organism and induced by the hormone-like substances.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12530584&dopt=Abstract

Braz J Med Biol Res. 2003 Feb;36(2):239-45. Epub 2003 Jan 29.
Gender-related differences in the effects of nitric oxide donors on neuroleptic-induced catalepsy in mice.

Pires JG, Costa PG, Saraiva FP, Bonikovski V, Futuro Neto HA.

Departamento de Ciencias Fisiologicas, Centro Biomedico, Universidade Federal do Espirito Santo, Vitoria, ES, Brasil. jgppirepd.ufes.br

It has been suggested that nigrostriatal dopaminergic transmission is modulated by nitric oxide (NO). Since there is evidence that gonadal hormones can affect extrapyramidal motor behavior in mammals, we investigated the effects of isosorbide dinitrate (ISD), linsidomine (SIN-1) and S-nitroso-N-acetylpenicillamine (SNAP), three pharmacologically different NO donors, on neuroleptic-induced catalepsy in 60- to 80-day-old male and female albino mice. Catalepsy was induced with haloperidol (1 mg/kg, ip) and measured at 30-min intervals by means of a bar test. Drugs (or appropriate vehicle) were injected ip 30 min before haloperidol, with each animal being used only once. ISD (5, 20 and 50 mg/kg) caused a dose-dependent inhibition of catalepsy in male mice (maximal effect 120 min after haloperidol: 64% inhibition). In the females only at the highest dose of ISD was an attenuation of catalepsy observed, which was mild and short lasting. SIN-1 (10 and 50 mg/kg) did not significantly affect catalepsy in female mice, while a significant attenuation was observed in males at the dose of 50 mg/kg (maximal inhibition: 60%). SNAP (20 mg/kg) significantly attenuated catalepsy in males 120 min after haloperidol (44% inhibition), but had no significant effect on females. These results basically agree with literature data showing that NO facilitates central dopaminergic transmission, although the mechanisms are not fully understood. They also reveal the existence of gender-related differences in this nitrergic modulation in mice, with females being less affected than males.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12563527&dopt=Abstract

J Endocrinol. 1999 Apr;161(1):107-13.
Comparison of mechanisms mediating uptake and efflux of thyroid hormones in the human choriocarcinoma cell line, JAR.

Mitchell AM, Rowan KA, Manley SW, Mortimer RH.

Conjoint Endocrine Laboratory, Royal Brisbane Hospital, Herston Road, Brisbane, Queensland 4029, Australia.

We compared the specificities of transport mechanisms for uptake and efflux of thyroid hormones in cells of the human choriocarcinoma cell line, JAR, to determine whether triiodothyronine (T3), thyroxine (T4) and reverse T3 (rT3) are carried by the same transport mechanism. Uptake of 125I-T3, 125I-T4 and 125I-rT3 was saturable and stereospecific, but not specific for T3, T4 and rT3, as unlabelled L-stereoisomers of the thyroid hormones inhibited uptake of each of the radiolabelled hormones. Efflux of 125I-T3 was also saturable and stereospecific and was inhibited by T4 and rT3. Efflux of 125I-T4 or 125I-rT3 was, in contrast, not significantly inhibited by any of the unlabelled thyroid hormones tested. A range of compounds known to interfere with receptor-mediated thyroid hormone uptake in cells inhibited uptake of 125I-T3 and 125I-rT3, but not 125I-T4. We conclude that in JAR cells uptake and efflux of 125I-T3 are mediated by saturable and stereospecific membrane transport processes. In contrast, the uptake, but not the efflux, of 125I-T4 and 125I-rT3 is saturable and stereospecific, indicating that uptake and efflux of T4 and rT3 in JAR cells occur by different mechanisms. These results suggest that in JAR cells thyroid hormones may be transported by at least two types of transporters: a low affinity iodothyronine transporter (Michaelis constant, Km, around 1 microM) which interacts with T3, T4 and rT3, but not amino acids, and an amino acid transporter which takes up T3, but not T4 or rT3. Efflux of T4 and rT3 appears to occur by passive diffusion in these cells.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10194534&dopt=Abstract

Arch Surg. 2002 Nov;137(11):1249-52.
Risk factors for lymph node metastases in breast ductal carcinoma in situ with minimal invasive component.

Wasserberg N, Morgenstern S, Schachter J, Fenig E, Lelcuk S, Gutman H.

Department of Surgery, Rabin Medical Center, Beilinson Campus, Petach Tikva, Israel.

HYPOTHESIS: Clinical and pathological variables may be predictors of axillary dissemination in T1mic and T1a breast carcinoma. DESIGN: Retrospective medical chart review. SETTING: University-affiliated tertiary referral center. PATIENTS: All patients diagnosed as having ductal carcinoma in situ (DCIS) with microinvasion between January 1, 1988, and December 30, 1998. MAIN OUTCOME MEASURES: Pathology slides were reviewed according to the 1997 Cancer Staging Manual put forth by the American Joint Committee on Cancer. The number of involved ducts was noted. Patients with no invasive component or invasive components larger than 5 mm were excluded. Pathological and clinical variables were analyzed for their effect on axillary lymph node metastases. RESULTS: The study group included 57 women aged 37 to 71 years (median, 60 years), 37 with T1mic disease and 20 with T1a. Modified radical mastectomy was performed in 29 patients (18 with T1mic and 11 with T1a) and breast-preserving surgery in 28 (19 with T1mic and 9 with T1a). Forty-three patients (28 with T1mic and 15 with T1a) underwent axillary lymph node dissection. Axillary involvement was detected in 3 patients in each group. Forty-seven patients received adjuvant therapy (radiotherapy alone, or with hormones or chemotherapy). Follow-up was 3 to 120 months (median, 40 months). One patient was unavailable for follow-up, another died of disseminated disease, and a third developed contralateral primary carcinoma. Comedo DCIS (P<.03) and the number of DCIS-involved ducts (P<.002) in the T1mic group, and nuclear grade 3 (P<.001) in both groups, were independent significant predictors of axillary metastases. CONCLUSIONS: The significant rate of axillary metastases in T1a and T1mic breast tumors makes axillary staging a must. High nuclear grade, comedo DCIS, and high number of DCIS-involved ducts may predict axillary metastasis and should be considered when axillary dissection is done selectively.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12413311&dopt=Abstract

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