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Arch Dermatol Res. 2003 Apr;295(1):19-23. Epub 2003 Feb 27.
A case of non-selective phagocytosis of hemosiderin and melanin of dermal histiocytes in stasis dermatitis.

Rivera MR, Ishihara M, Mihara M.

Department of Dermatology, Faculty of Medicine, Tottori University, 36-1 Nishimachi, 683-8504 Yonago, Japan.

A case study was undertaken to determine whether or not the same dermal histiocytes could phagocytose both melanin and hemosiderin simultaneously. A biopsy specimen was taken from a pigmented lesion of the lower leg of a 57-year-old woman with stasis dermatitis. The specimen was processed for histology, conventional transmission electron microscopy and electron-probe X-ray microanalysis. Histologically, numerous histiocytes with their cytoplasm packed with either Prussian blue-positive granules or Fontana-Masson-positive granules were distributed almost equally in the dermis. Electron microscopically, the dermis had many histiocytes with their cytoplasm containing solitary or compound electron-dense substances. The electron-dense substances were classified into three types according to their degree of electron density. By electron-probe X-ray microanalysis, these electron-dense substances were classified into iron-containing and non-iron-containing substances. Both substances were seen in the cytoplasm of the same histiocytes and even in the same compound electron-dense substance. The former were siderosomes and the latter were probably melanosomes. These results show that the same dermal histiocytes probably phagocytose non-selectively both hemosiderin and melanin granules.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12709816&dopt=Abstract [PubMed - in process]



Heredity. 2003 May;90(5):359-64.
Genetic and environmental components of variation in eumelanin and phaeomelanin sex-traits in the barn owl.

Roulin A, Dijkstra C.

1University of Cambridge, Department of Zoology, Downing Street, CB2 3EJ Cambridge, UK.

Knowledge of the mechanism underlying the expression of melanin-based sex-traits may help us to understand their signalling function. Potential sources of inter-individual variation are the total amount of melanins produced but also how biochemical precursors are allocated into the eumelanin and phaeomelanin pigments responsible for black and reddish-brown colours, respectively. In the barn owl (Tyto alba), a eumelanin trait (referred to as 'plumage spottiness') signals immunocompetence towards an artificially administrated antigen and parasite resistance in females, whereas a phaeomelanin trait ('plumage coloration') signals investment in reproduction in males. This raises the question whether plumage coloration and spottiness are expressed independent of each other. To investigate this question, we have studied the genetics of these two plumage traits. Crossfostering experiments showed that, for each trait, phenotypic variation has a strong genetic component, whereas no environmental component could be detected. Plumage coloration is autosomally inherited, as suggested by the similar paternal-to-maternal contribution to offspring coloration. In contrast, plumage spottiness may be sex-linked inherited (in birds, females are heterogametic). That proposition arises from the observation that sons resembled their mother more than their father and that daughters resembled only their father. Despite plumage coloration and spottiness signalling different qualities, these two traits are not inherited independent of each other, darker birds being spottier. This suggests that the extent to which coloration and spottiness are expressed depends on the total amount of melanin produced (with more melanin leading to a both darker and spottier plumage) rather than on differential allocation of melanin into plumage coloration and spottiness (in such a case, darker birds should have been less spotted). A gene controlling the production of melanin pigments may be located on sex-chromosomes, since the phenotypic correlation between coloration and spottiness was stronger in males than in females.Heredity (2003) 90, 359-364. doi:10.1038/sj.hdy.6800260


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12714980&dopt=Abstract [PubMed - in process]



J Cosmet Sci. 2003 Mar-Apr;54(2):133-42.
Inhibitory effects of Ramulus mori extracts on melanogenesis.

Lee KT, Lee KS, Jeong JH, Jo BK, Heo MY, Kim HP.

Coreana Cosmetics Co., 204-1 JeongchonRi, Senggeoeup, Cheonansi, 330-830, Korea.

To develop an active agent for skin whitening, the inhibitory effects of 285 plant extracts on tyrosinase activity were examined, and one plant extract having tyrosinase inhibition activity was chosen. Ramulus mori (young twigs of Morus alba L.) extracts showed inhibition activity in tyrosinase and melanin synthesis in B-16 melanoma cells. To clarify the mechanism of its inhibition on melanogenesis, the effect of R. mori extracts on tyrosinase activity, synthesis, and gene expression was evaluated. R. mori extracts showed tyrosinase inhibition activity by competitive method, and there was no suppression of tyrosinase synthesis and gene expression. Further, to evaluate the inhibitory activity of R. mori in vivo, its effect on melanin production in UV-induced brown guinea pigs was examined, where a decrease of melanin production in the guinea pig model was observed. Also, R. mori extracts showed no toxicity in animal tests such as the acute toxicity test, the skin irritation test, the eye irritation test, the skin sensitization test, and the acute oral toxicity test, and no toxicity in the human skin irritation test. A single compound from R. mori extracts was purified using various column chromatography and recrystallization, and its chemical structure was identified using mass chromatography, IR spectroscopy, and NMR analysis. The chemical structure was that of 2,3',4,5'-tetrahydroxystilbene(2-oxyresveratrol) and showed inhibition activity on tyrosinase (IC(50) = 0.23 microg/ml). Also, R. mori extracts inhibited tyrosinase activity in a competitive manner (Ki = 1.5 x 10(-6) M) when L-tyrosine was used as a substrate.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12715091&dopt=Abstract







Natural Herbal Supplement: Hair Million


Hair loss alone does not pose significant health problems. In fact, there are people who opt for baldness as an alternative hair style. However, in general, however, hair loss is not considered desirable.

The most ostensive feature that distinguishes us human from chimps and other primates is the lack of bodily hair. During evolutionary process, we have lost the majority of hair. Hair is no longer a biologically essential part of our body, just like appendix. The hair we still have on our scalp and a few other bodily parts is still regarded as significant for reasons other than biological necessity. Hair loss is naturally accompanied by aging process, although the extent of hair loss and the timing of onset vary widely among individuals. Thus, loss of hair and baldness is considered as a symbol of maturity or old age. Like winkles and other signs of aging, hair loss is not welcome by most people, because we don't welcome aging, and being perceived as an aging person. However, it is alopecia, or premature hair loss that especially concerns certain people.

While the hair loss and resulting baldness in general have not been proven to be related to underlying health problems, there are certain correlations between hair loss and health problems. For instance, premature hair loss could suggest premature aging or nutritional and hormonal imbalance, stressful life, use of drugs that cause hair loss as a side effect, skin disease, or heart disease. The balding appearance could also impart a subdued impression of integrity in bodily health and youthfulness.












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