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Interferon research abs 1 || Hemoglobin research abs || Stem cell research abs || Nucleic acid research abs || Herpes research abs || Bronchitis research abs || Schizophrenia research abs || Tuberculosis research abs || Pneumonia research abs || Constipation research abs || Laxative research abs || hair research abs || hair related research references || testosterone related research references || melanin related research references

Acta Derm Venereol. 1976;56(3):223-8.
Effects of dibutyryl adenosine 3',5'-cyclic monophosphate on human melanocytes in vitro.

Kitano Y.

The effects of cyclic AMP and its analogue, dibutyryl cyclic AMP (DBcAMP) on the pigmentary system were studied by using human epidermal melanocytes in culture. The melanocytes responded to 1 mM DBcAMP with an increase in number, length, and complexity of dendritic processes. The effect of DBcAMP on the dendritogenesis was reversible. Melanin synthesis, as indicated by the uptake of tyrosine in the presence of an inhibitor of protein synthesis, was significantly stimulated by DBcAMP. The maximum stimulation was observed at concentrations of 0.5 mM, and 1.0 mM. The melanin synthesis increased after 12-hour treatment with DBcAMP and continued to increase with the prolonged treatment. Cyclic AMP, theophylline, sodium butyrate, or 5'-AMP at a concentration of 1 mM did not have any remarkable effect on the morphology or the melanin synthesis of the melanocyte. The results of this investigation indicate the possible role of the MSH-cyclic AMP system in the melanin pigmentation of human skin and represent a system for further study of the pathobiology of human melanocytes.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=59507&dopt=Abstract

Histochemistry. 1977 Mar 4;51(2-3):141-52.
Reduction and azo coupling of quinones. A histochemical study of human cutaneous melanin and adrenochrome.

Lillie RD, Donaldson PT, Vacca LL, Pizzolato PP, Jirge SK.

Cutaneous melanin in formol fixed skin and adrenochrome in dichromate fixed monkey adrenal after adequate bisulfite or dithonite reduction were found to give definite azo coupling reactions. Weaker reactions were obtained on unreduced material, and these disappeared on ferric chloride oxidation. Both cutaneous melanin and adrenochrome appear to exist in a quinhydrone status. Prolongation of dichromate treatment weakens or abolishes azo coupling capacity of adrenochrome. The findings support the concept of quinonization and reduction to prevent and restore azo coupling of enterochromaffin cells and noradrenaline islets of the adrenal. The most effective diazos for melanin were p-nitrodiazobenzene, fast black K and the diazosulfanilic acid, pH 1 pyronin B procedure, for adrenochrome. Diazosafranin and 2-chloro-4-nitrodiazobenzene were also useful. Blue and violet coupling products from toluidine blue and methylene violet RR fail to yield sufficient contrast to be convincing.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=66222&dopt=Abstract

Acta Otolaryngol. 1977 Jul-Aug;84(1-2):24-37.
Pigmentation of the stria vascularis. The contribution of neural crest melanocytes.

Hilding DA, Ginzberg RD.

Because all stages of melanogenesis (premelanosome, melanosome and melanin granules) were found in intermediate cells of the rat stria vascularis, they can be classified as melanocytes. The marginal and basal cells of these rat specimens contained no pigment. Early in development, the melanocytes or future intermediate cells are located beneath the strial basal lamina. They penetrate this lamina to insert themselves between marginal cells. Both the melanocytes and marginal cells are believed to participate actively in the tissue rearrangements which ultimately bring the two cell types into intimate structural relationship. The ingrowing melanocytes and the mature, pigmented, intermediate cells which they form are frequently associated with blood vessels. Pretreatment with Dopa to enhance the melanin content of early melanocytes did not enable us to identify their route of migration into the stria.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=70955&dopt=Abstract

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