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Ann Pathol. 2000 Dec;20(6):643-5.
[Resins polymerizing at low temperatures. I. Different families and principle technics]

[Article in French]

Ait Ouazzou S, Daneshpouy M, Rivet J, Meignin V, Plockyn A, Janin A.

Service d'Anatomie Pathologique et Laboratoire UPRES EA2378. Hopital Saint-Louis, 1, avenue Claude Vellefaux, 75475 Paris Cedex 10.

For pathologists, tissue processing and analysis require good preservation of both the shape (morphology) and the content of the cells (antigens, nucleic acids). Low temperature embedding resins are the only inclusion substrate which allows both a fine morphological analysis and good preservation of antigens and nucleic acids. Automatic the technical processes and simplified protocols now allow the introduction of low temperature embedding resins in diagnostic procedures.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11148368&dopt=Abstract

Dev Growth Differ. 2001 Feb;43(1):13-23.
Primary structure and developmental expression of Bufo arenarum cellular nucleic acid-binding protein: changes in subcellular localization during early embryogenesis.

Armas P, Cabada MO, Calcaterra NB.

Division Biologia del Desarrollo, IBR, CONICET - Area de Biologia General, Dpto. de Ciencias Biologicas, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha 531 (2000) Rosario, Republica Argentina.

A Bufo arenarum cellular nucleic acid-binding protein (bCNBP) full-length cDNA was cloned. bCNBP is a 19.4 kDa protein containing seven CCHC zinc finger motifs, an RGG box and a Ser-rich region. Amino acid comparisons showed high values of homology in vertebrates and smaller values in insects or inferior eukaryotes. Northern blot analysis during oogenesis and early development revealed two transcripts with different expressions of pattern behavior. One of them is present in all stages analyzed, whereas the other is only detected from the beginning of zygotic transcription. Immunocytochemistry assays carried out on sections of ovary and early embryos showed that there was no specific staining of previtellogenic oocytes. In early vitellogenic oocytes, in oocytes at stages V/VI and in embryos at early blastula stage, reaction was observed inside the cytoplasm. At mid-blastula stage, CNBP was mainly detected in the epiblast. At the late gastrula stage, two layers of cells were stained in the archenteron roof, in which the internal one presented as strong staining. Nuclei in this layer were stained even stronger than the cytoplasm. Changes in mRNA expression patterns, accompanied by changes in subcellular localization, suggest that CNBP might interact with both nuclear and cytoplasmic nucleic acids.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11148448&dopt=Abstract

Pathol Int. 2001 Jan;51(1):55-9.
Blastic natural killer cell lymphoma arising from the mediastinum with terminal deoxynucleotidyl transferase expression.

Isobe K, Tamaru JI, Nakamura S, Itami J, Aruga T, Uno T, Yasuda S, Mikata A, Ito H.

Department of Radiology, Chiba University, School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.

Blastic natural killer (NK) cell lymphoma/leukemia is a relatively rare NK cell malignancy. We report the second case of blastic NK cell lymphoma arising from the mediastinum with an aggressive clinical course. The patient was a 63-year-old Japanese man with an anterior mediastinum tumor. The biopsy specimen showed diffuse proliferation of tumor cells with frequent mitotic figures and apoptotic bodies. Both angiocentric features and small foci of coagulative necrosis were found in this section. The tumor cells had medium to large nuclei with a fine chromatin pattern, inconspicuous nucleoli and scanty cytoplasm. The nuclear contour was oval to moderately irregular, showing slight pleomorphism as compared with typical lymphoblastic lymphoma. The tumor cells were positive for CD2, CD56 and terminal deoxynucleotidyl transferase, but negative for other T-cell antigens, B-cell antigens and myeloid markers. In situ hybridization for Epstein-Barr virus encoded small ribonucleic acid 1 was negative.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11148466&dopt=Abstract

Arh Hig Rada Toksikol. 2000 Sep;51(3):335-41.
Bioactivity and analysis of chiral compounds.

Kezic S.

Coronel Institute of Occupational and Environmental Health, University of Amsterdam, Academic Medical Centre, The Netherlands. S.Kezimc.uva.nl

The stereochemistry of drug and xenobiotic metabolism and toxicokinetics have recently become an issue for the pharmaceutical industry and the regulatory authorities. Chirality is an intrinsic property of macromolecular structures in the cells such as enzymes, receptors, and nucleic acids which becomes evident when they interact with a chiral drug or xenobiotic molecule, showing a high degree of stereoselectivity. There are now a range of examples of isomeric compounds whose biological activity may well reside predominantly in one enantiomer. The differences in the biological activities between enantiomers have resulted in an increased interest in determination of enantiomeric composition of drugs, xenobiotics, and/or their metabolites. The difficulty in determining enantiomeric excess arises from the fact that enantiomers have identical physicochemical properties in a non-chiral environment. The major analytical breakthrough has come with the emergency of commercially available chiral stationary phases for capillary gas chromatography, high-performance liquid chromatography, and capillary electrophoresis.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11148938&dopt=Abstract

J Biomol Struct Dyn. 2000 Dec;18(3):353-62.
Computational procedures to explain the different biological activity of DNA/DNA, DNA/PNA and PNA/PNA hybrid molecules mimicking NF-kappaB binding sites.

Saviano M, Romanelli A, Bucci E, Pedone C, Mischiati C, Bianchi N, Feriotto G, Borgatti M, Gambari R.

Biocrystallography Research Centre, CNR, Napoli, Italy. savianhemistry.unina.it

Peptide nucleic acids (PNA) have recently been proposed as alternative reagents in experiments aimed to the control of gene expression. In PNAs, the pseudopeptide backbone is composed of N-(2-aminoethyl)glycine units and therefore is stable in human serum and cellular extracts. PNAs hybridize with high affinity to complementary sequences of single-stranded RNA and DNA, forming Watson-Crick double helices and giving rise to highly stable (PNA)2-RNA triplexes with RNA targets. Therefore, antisense and antigene PNAs have been synthetized and characterized. The major issue of the present paper is to describe some computational procedures useful to compare the behaviour of PNA double stranded molecules and PNA/DNA hybrids with the behaviour of regular DNA duplexes in generating complexes with DNA-binding proteins. The performed computational analyses clearly allow to predict that the lack of charged phosphate groups and the different shape of helix play a critical role in the binding efficiency of NF-kappaB transcription factors. These computational analyses are in agreement with competitive gel shift and UV-cross linking experiments. These experiments demonstrate that NF-kappaB PNA/PNA hybrids do not interact efficiently with proteins recognizing the NF-kappaB binding sites in genomic sequences. In addition, the data obtained indicate that the same NF-kappaB binding proteins recognize both the NF-kappaB DNA/PNA and DNA/DNA hybrids, but the molecular complexes generated with NF-kappaB DNA/PNA hybrids are less stable than those generated with NF-kappaB target DNA/DNA molecules.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11149512&dopt=Abstract

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