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Clin Hemorheol Microcirc. 2002;27(2):115-22.
Exercise-induced central retinal vein thrombosis: possible involvement of hemorheological disturbances. A case report.

Gaudard A, Varlet-Marie E, Monnier JF, Janbon Ch, Quere I, Bressolle F, Brun JF.

Laboratoire de Pharmacocinetique Clinique, Faculte de Pharmacie, Universite Montpellier I, France.

Exercise-induced impairment in blood fluidity has been supposed to increase cardiovascular risk but there is no data to support this hypothesis. We report the case of a 50 yr old marathon runner who underwent a central retinal vein thrombosis after a marathon run. We investigated his rheological response to exercise compared to control subjects of previous studies. During a standardized sub-maximal exercise-test, the increase in blood viscosity (+28%) and hematocrit (+25%) exceeded the control range but the most striking differences were found for red cell aggregation (Myrenne +47%) and disaggregation thresholds (Affibio +37%). Although some of this post-exercise hyperviscosity pattern may be due to the previous vascular event, these findings may also support the hypothesis of a role for hemorheological alterations during exercise in the pathogenesis of this marathon-induced retinal thrombosis, and indicate that after such an event hemorheological adaptation to exercise remains markedly disturbed.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12237481&dopt=Abstract

J Vet Med Sci. 2002 Aug;64(8):739-41.
Production and characterization of biodegradable Povidone-iodine microsphere as a intramammary disinfectant.

Park HM, Han HR.

Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Korea.

Microspheres composed of biocompatible, biodegradable poly DL-lactide-co-glycolide (DL-PLGA) and Povidone-iodine were evaluated as an intramammary disinfectant delivery system in vitro prior to infusion into mammary glands. Microsphere was prepared by solvent evaporation method and particle size, morphology and in vitro release kinetics were examined. The microspheres were ranged in size from 25 microm to 155 microm (mean diameter = 65.7 microm). Povidone-iodine was dispersed on the surface of microsphere and microsphere was spherical in shape with a smooth surface. The yield of microsphere was 57.3% and the encapsulation efficiency was 69.6%. In in vitro release study, a burst effect (50.9%) was observed during the first two days and a sustained release then continued for the next 28 days. Results of the present study demonstrated that microsphere have the potential for new intramammary disinfectant formulations that can provide increased efficacy of therapy against mastitis pathogens.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12237524&dopt=Abstract

J Neurochem. 2002 Dec;83(6):1431-40.
Astrocytic but not neuronal increased expression and redistribution of parkin during unfolded protein stress.

Ledesma MD, Galvan C, Hellias B, Dotti C, Jensen PH.

Universita degli Studi di Torino, Cavalieri Ottolenghi Scientific Institute, Torino, Italy.

Parkin is a ubiquitin ligase that facilitates proteasomal protein degradation and is involved in a common autosomal recessive form of Parkinson's disease. Its expression is part of the unfolded protein response in cell lines where its overexpression protects against unfolded protein stress. How parkin expression is regulated in brain primary cells under stress situations is however, less well established. Here, the cellular and subcellular localization of parkin under basal conditions and during unfolded protein stress was investigated in primary cultures of rat astrocytes and hippocampal neurons. Immunofluorescense microscopy and biochemical analysis demonstrated that parkin is mainly associated with the endoplasmic reticulum (ER) in hippocampal neurons while it is associated with Golgi membranes, the nuclei and light vesicles in astrocytes. The constitutive parkin expression was high in neurons as compared with astrocytes. However, unfolded protein stress elicited a selective increase in astrocytic parkin expression and a change in distribution, whereas neuronal parkin remained largely unmodified. The cell specific differences argue in favour of different cellular binding sites and substrates for the protein and a pathogenic role for astrocytes in Parkinson's disease caused by parkin dysfunction.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12472897&dopt=Abstract

Pediatr Infect Dis J. 2002 Jul;21(7):623-8.
Foodborne disease outbreaks in United States schools.

Daniels NA, MacKinnon L, Rowe SM, Bean NH, Griffin PM, Mead PS.

Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. ndanieledicine.ucsf.edu

BACKGROUND: The objective of this study was to describe the epidemiology of foodborne disease outbreaks in schools and to identify where preventive measures could be targeted. METHODS: Reports by state and local health departments of foodborne disease outbreaks occurring in primary and secondary schools, colleges and universities from January 1, 1973, through December 31, 1997, were reviewed. Data from ill persons identified through foodborne outbreak investigations and subsequently reported to the Centers for Disease Control and Prevention in the Foodborne Outbreak Surveillance System were examined. The number and size of foodborne disease outbreaks, as well as the etiologic agents, food vehicles of transmission, site of food preparation and contributing factors associated with outbreaks were also examined. RESULTS: From 1973 through 1997, states and local health departments reported 604 outbreaks of foodborne disease in schools. The median number of school outbreaks annually was 25 (range, 9 to 44). In 60% of the outbreaks an etiology was not determined, and in 45% a specific food vehicle of transmission was not determined. Salmonella was the most commonly identified pathogen, accounting for 36% of outbreak reports with a known etiology. Specific food vehicles of transmission were epidemiologically identified in 333 (55%) of the 604 outbreaks. The most commonly implicated vehicles were foods containing poultry (18.6%), salads (6.0%), Mexican-style food (6.0%), beef (5.7%) and dairy products excluding ice cream (5.0%). The most commonly reported food preparation practices that contributed to these school-related outbreaks were improper food storage and holding temperatures and food contaminated by a food handler. CONCLUSIONS: Strengthening food safety measures in schools would better protect students and school staff from outbreaks of foodborne illness. Infection control policies, such as training and certification of food handlers in the proper storage and cooking of foods, meticulous hand washing and paid sick leave for food handlers with gastroenteritis, could make meals safer for American students.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12237592&dopt=Abstract

Pediatr Infect Dis J. 2002 Jul;21(7):636-41.
Evaluation of the role of respiratory viruses in acute myringitis in children less than two years of age.

Kotikoski MJ, Palmu AA, Nokso-Koivisto J, Kleemola M.

Tampere University Hospital, Finland.

BACKGROUND: The etiology of acute myringitis remains controversial although it is usually encountered in connection with acute otitis media (AOM). In most cases of acute myringitis a bacterial pathogen has been detected in the middle ear fluid, but the role of respiratory viruses has remained unclear. Our objective was to investigate the etiologic role of viruses in the pathogenesis of acute bullous and hemorrhagic myringitis in children <2 years of age. METHODS: A prospective longitudinal cohort study of 2028 children ages 7 to 24 months in primary care in the Finnish Otitis Media Vaccine Trial. Nasopharyngeal aspirate (NPA) and middle ear fluid (MEF) samples taken at the time of the diagnosis were examined by a time-resolved fluoroimmunoassay for antigen detection of adenoviruses; influenza viruses A and B; parainfluenza viruses 1, 2 and 3; and respiratory syncytial virus and by reverse transcription polymerase chain reaction for human rhinovirus and human enterovirus. RESULTS: Eighty-two children with bullous myringitis and 37 children with hemorrhagic myringitis were diagnosed during the 18-month follow-up period. In bullous myringitis a respiratory virus was detected in 70% of NPA samples and in 27% of MEF samples. In hemorrhagic myringitis 57% of NPA samples and 28% of MEF samples were virus-positive. The viral distribution was similar to that of AOM (virus positive 64% of NPA and 37% of MEF). CONCLUSIONS: We could not confirm any specific respiratory virus to be the etiologic agent in acute myringitis. The etiology of acute myringitis is similar to that of AOM in children <2 years of age.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12237595&dopt=Abstract

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