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Fatty acids resources:

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Acta Crystallogr D Biol Crystallogr. 2000 Nov;56 ( Pt 11):1452-5.
Crystallization and preliminary X-ray diffraction analysis of a functional form of pneumolysin, a virulence factor from Streptococcus pneumoniae.

Kelly SJ, Jedrzejas MJ.

Department of Microbiology, University of Alabama at Birmingham, AL 35294, USA.

Pneumolysin is a virulence factor from Streptococcus pneumoniae, a Gram-positive bacterial pathogen which causes human infections with a severe impact on mortality and morbidity worldwide. The enzyme belongs to a group of cholesterol-dependent cytolysins and interacts with its cholesterol receptor on target cells, leading to pneumolysin insertion into target-cell membranes and subsequently to pore formation and cell lysis. Pneumolysin has been overexpressed, purified and crystallized for X-ray diffraction studies. Crystals have been obtained in the presence of cholesterol in an effort to produce a three-dimensional structure of pneumolysin in its fully functional form with the enzyme bound to its activator. This is the first report of the crystallization of a cholesterol-dependent cytolysin in the presence of bound cholesterol. The vapor-diffusion method using ammonium sulfate as a precipitation agent was used to grow crystals in the presence of n-octyl-beta-D-glucopyranoside and phosphatidylcholine. Crystals of this 53 kDa molecule complexed with cholesterol diffracted X-rays to 3.3 A. The crystal unit cell has parameters a = b = 191.45, c = 66.16 A, alpha = beta = 90.0, gamma = 120 degrees and belongs to the trigonal space group P3. The determination of the three-dimensional structure of this pneumococcal cytolysin is in progress.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11053849&dopt=Abstract



Acta Crystallogr D Biol Crystallogr. 2000 Nov;56 ( Pt 11):1482-4.
Crystallization and preliminary X-ray diffraction studies of a new crystal form of human secretory type IIA phospholipase A2.

Church WB, Lei PW, Ogg DJ, Scott KF.

Arthritis and Inflammation Research Program, The Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, NSW 2010, Australia. b.churcarvan.unsw.edu.au

Human synovial type IIA phospholipase A(2) (sPLA(2)-IIA) has been implicated in the pathogenesis of a number of inflammatory diseases and is a target for the development of therapeutically useful inhibitors. Biochemical evidence suggests a novel mechanism of inhibition for a series of peptide inhibitors originally derived from the primary sequence of the protein. On co-incubation with one of these inhibitors, single crystals of a hitherto unreported crystallographic form of sPLA2-IIA suitable for diffraction analysis were obtained. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 140.8, b = 38.9, c = 109.1 A, beta = 125.1 degrees, and diffraction at 2.4 A resolution has been observed.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11053860&dopt=Abstract



Acta Crystallogr D Biol Crystallogr. 2000 Nov;56 ( Pt 11):1495-7.
Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea.

Liao DI, Viitanen PV, Jordan DB.

DuPont Central Research and Development, Experimental Station, Wilmington, Delaware 19880, USA.

Dihydroxybutanone phosphate synthase (DS) catalyzes a commitment step in riboflavin biosynthesis where ribulose 5-phosphate is converted to dihydroxybutanone phosphate and formate. DS was cloned from the pathogenic fungus Magnaporthe grisea (using functional complementation of an Escherichia coli DS knockout mutant) and expressed in E. coli. The purified protein crystallized in space group P2(1)2(1)2. Diffraction data extending to 1.5, 1.0 and 1.8 A resolution were collected from crystals that were divalent cation free, soaked in Zn(2+) or soaked in Mg(2+), respectively.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11053863&dopt=Abstract



Acta Crystallogr D Biol Crystallogr. 2000 Nov;56 ( Pt 11):1501-4.
The purification, crystallization and preliminary structural characterization of glucose-1-phosphate thymidylyltransferase (RmlA), the first enzyme of the dTDP-L-rhamnose synthesis pathway from Pseudomonas aeruginosa.

Blankenfeldt W, Giraud MF, Leonard G, Rahim R, Creuzenet C, Lam JS, Naismith JH.

Centre for Biomedical Sciences, North Haugh, The University, St Andrews, Fife KY16 9ST, Scotland.

Glucose-1-phosphate thymidylyltransferase (RmlA; E.C. 2.7.7.24) is the first of four enzymes involved in the biosynthesis of dTDP-L-rhamnose, the precursor of L-rhamnose, a key component of the cell wall of many pathogenic bacteria. RmlA catalyses the condensation of thymidine triphosphate (dTTP) and alpha-D-glucose-1-phosphate (G1P), yielding dTDP-D-glucose. RmlA from Pseudomonas aeruginosa has been overexpressed and purified. Crystals of the enzyme have been grown using the sitting-drop vapour-diffusion technique with PEG 6000 and lithium sulfate as precipitant. Several diffraction data sets of single frozen crystals were collected to a resolution of 1.66 A. Crystals belonged to space group P1, with unit-cell parameters a = 71.5, b = 73.1, c = 134.7 A, alpha = 89.9, beta = 80.9, gamma = 81.1 degrees. The asymmetric unit contains eight monomers in the form of two RmlA tetramers with a solvent content of 51%. Selenomethionine-labelled protein has been obtained and crystallized.


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11053865&dopt=Abstract



Dermatology. 2000;201(2):146-7.
High prevalence of seborrhoeic dermatitis on the face and scalp in mountain guides.

Moehrle M, Dennenmoser B, Schlagenhauff B, Thomma S, Garbe C.

Department of Dermatology, University of Tubingen, Germany. matthias.moehrled.uni-tuebingen.de

BACKGROUND: High incidence rates of seborrhoeic dermatitis (SD) have been reported in HIV-infected individuals, indicating immunosuppression to be involved in the pathogenesis. OBJECTIVE: To establish the prevalence of SD in mountain guides who have a high occupational exposure to solar UV radiation. PATIENTS AND METHODS: In November 1999, 283 mountain guides were physically examined on the face and scalp for symptoms of SD in Austria (n = 75), Switzerland (n = 123) and Germany (n = 85); they were 21.3-93.1 years of age (median age 41.4 years). RESULTS: Forty-six of 283 (16. 3%) mountain guides when examined clinically were found to have SD. The median age of mountain guides with SD was 41.2 years. There were similar incidence rates in all three countries. CONCLUSION: SD affects mountain guides in a clearly higher percentage as the general population. We suggest UV-induced immunosuppression due to occupational sun exposure as a pathogenetic factor. 2000 S. Karger AG, Basel


online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11053918&dopt=Abstract








Like developmental biology of any part of our body, hair growth is a complicated process. Hence the homework for modern science to yet unravel the process and mechanism to a completion. There exist a number of traditional and alternative therapeutic methods that include drugs, surgery, suppelements, and even snake oils that have been developed and used for those who lose hair. No understanding, and there is no solution. Of course, none of these approaches are perfect for all hair loss problems, especially due to the heterogeneity of the causes underlying hair losses. Most of chemical drugs and hair transplantation surgeries are accompanied by undesirable side effects.
















DHEA is a natural hormone, and it is produced in our body by the adrenal glands. DHEA has been suggested to provide numerous potential benefits. DHEA (or dehydroepiandrosterone) is converted into androgens (male hormones) or estrogens (female hormones) in the cells. Our bodies produce decreasing amount of DHEA as we get older. various health benefits: To deter aging, improve sexual function/erectile dysfunction, treat cognitive decline, enhance athletic performance, facilitate weight loss, improve strength, prevent osteoporosis, enhance immunomodulation for rheumatic conditions, and treat depression.







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