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Trop Anim Health Prod. 2000 Oct;32(5):277-84.
Response of chickens to infection with Newcastle disease virus isolated from a guinea fowl.

Mishra S, Kataria JM, Verma KC, Sah RL.

Division of Health, CSWRI, Rajasthan, India.

An isolate of Newcastle disease virus obtained from a guinea fowl was characterized as a viscerotropic velogenic strain based upon pathogenicity index studies. Following inoculation of the viral isolate oronasally into 3-week-old chickens, clinical signs appeared after an incubation period of 4-5 days and included dullness, depression, dyspnoea, diarrhoea and leg paralysis. The virus caused a mortality of 56% with haemorrhages at the tip of the glands of the proventriculus and caecal tonsil. Histopathological changes were prominent in the lymphoid organs, being characterized by depletion, degeneration and necrosis of the lymphoid tissues. The brain was the first organ affected, with changes being noticed 3 days after infection. Isolation of virus from various organs was more frequent from 5 to 10 days after infection, but the virus persisted in some of the organs until 21 days after infection. In spite of the high mortality, a good immune response was elicited by the isolate, as was evident from the antibody titre.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11059036&dopt=Abstract

Ann Ital Med Int. 2000 Jul-Sep;15(3):226-31.
[Costal osteolytic lesions and multiple hepatic neoformations in a patient with inflammatory pseudotumor]

[Article in Italian]

Gabrielli GB, Casaril M, Stanzial AM, Corrocher R.

Dipartimento di Medicina Clinica e Sperimentale, Universita degli Studi di Verona.

Inflammatory pseudotumor is a rare disease, that is regarded as a benign reactive inflammatory process, although its etiology and pathogenesis are still unknown. The liver is one of the organs most frequently involved, but inflammatory pseudotumors have been reported in many other sites in the body. Inflammatory pseudotumor of the liver presents as a solitary or, less frequently, multiple space-occupying lesion, which the common imaging techniques do not clearly distinguish from primitive or metastatic hepatic malignancies. Biopsy of the lesion is therefore necessary for diagnosis. The case of inflammatory pseudotumor described here presented with radiologic features of multiple solid space-occupying lesions in the liver, associated with multiple osteolytic lesions in the ribs. Such an association, very suggestive of malignancy, has not yet been reported for inflammatory pseudotumors. Optimum management of this disease has not yet been standardized. The majority of patients are treated by hepatic resection, although spontaneous regression has also been described. In our case, rapid improvement of both hepatic and costal lesions was observed, although the patient did not receive any specific treatment.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11059065&dopt=Abstract

Bratisl Lek Listy. 2000;101(7):365-70.
Alterations in lymphocyte subpopulations in peripheral blood at manifestation of type 1 diabetes mellitus in childhood.

Michalkova D, Mikulecky M, Tibenska E.

1st Department of Pediatry, University Hospital DFN, Diabctological Center Slovak Republic, Bratislava. michalkmed.uniba.sk

BACKGROUND: Alterations in cellular immunity at manifestation of type 1 diabetes mellitus, as described in publications so far, are equivocal. Moreover, the age of children was usually not taken into account. OBJECTIVES: Exact inferentially statistical measures were used to arrive at reliable information. METHODS: Thirty four diabetic children and 48 normals were taken randomly according to the established criteria, and scrutinized. Lymphocyte subpopulations counts were measured by flow cytometry using three-color-labelled monoclonal antibodies against cell surface markers. The resulting absolute cell counts as well as percentages from the total lymphocyte count were expressed in terms of univariate and bivariate 95% confidence intervals. They render an illustrative way for defining statistically significant (alpha = 5%) differences between health and disease. RESULTS: The CD8, CD16 absolute counts in younger diabetics were significantly decreased in average to 96-58% of the normal subgroup. For older children, CD4, CD8, CD16 and CD19 absolute counts were significantly lowered to 75-61% of the norm. Relative changes in Ly subpopulations were less pronounced. The immunoregulatory index increased significantly to 125-128% of the norm in either age group. The proportion of CD4 memory cells from the total of naive and memory cells was significantly increased to 122-133% of the norm in diabetic children of either age group. CONCLUSION: More significant changes of lymphocyte subpopulations than those given in literature were revealed at manifestation of childhood type 1 diabetes. They testify to the autoimmune pathogenesis of the type 1 diabetes mellitus. (Tab. 3, Fig. 4, Ref. 18.)

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11059105&dopt=Abstract

Minerva Chir. 2000 Jun;55(6):451-3.
[Ischemic pathogenesis of acute pancreatitis. A case report]

[Article in Italian]

Nistri R, Vitali A, Basili G, Carrieri P.

U. O. Patologia Chirurgica II, Universita degli Studi, Firenze.

An ischemic origin of acute pancreatitis has been considered for a long time, at least as an aiding factor: ischemia has a fundamental role in the development of necrotizing pancreatitis from an oedematous one. Shock, heart condition and celiac-mesenteric ischemia can determine the onset of an acute pancreatitis through local ischemic lesions. Personal experience with a case of acute pancreatitis following an intestinal ischemic failure is reported. The duration of ischemia and, in particular, the free radicals formation during the organ reperfusion have been considered as the main pathogenetic factors. In the observed case, the ischemic hypothesis seems to be supported from the lack of other known factors and from the intestinal ischemic failure episode.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11059241&dopt=Abstract

Minn Med. 2000 Oct;83(10):53-8.
Antibiotic bacterial resistance in ambulatory patients.

Yawn BP, Wollan P, Cockerill F, Lydick E.

Olmsted Medical Center Department of Research, USA.

BACKGROUND: This study evaluates trends in antibiotic resistance in patients who were treated in an ambulatory setting. METHODS: The authors compiled the data from all lower respiratory track(sputum) cultures collected from ambulatory patients who visited the Olmsted Medical Center and Mayo Clinic between 1985 and 1998. Cultured organisms were identified, and Minimal Inhibitory Concentration (MIC) values were presented and categorized as susceptible, intermediate, or resistant based on the National Committee for Clinical Laboratory Standards (NCCLS) guidelines for MIC and antibiotic susceptibility. RESULTS: 4,297 potentially pathogenic organisms were obtained from sputum cultures for 1,921 patients. The most discernible changes in antibiotic resistance appeared to be in cultures positive for Pseudomonas aeruginosa. A trend toward increasing resistance of isolates of Streptococcus pneumoniae to beta-lactam drugs was observed in a portion of the population. An emerging intermediate susceptibility among isolates of Klebsiella pneumoniae and Pseudoumonas species was noted. CONCLUSIONS: Trends in antimicrobial resistance of respiratory pathogens from ambulatory patients are less clear than those from hospitalized patients, but must be monitored because of the high percentage of ambulatory patients who receive empirical therapies. Trends in intermediate susceptibility patterns may help reveal emerging antimicrobial resistance.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11059252&dopt=Abstract

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