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Interferon research abs 1 || Hemoglobin research abs || Stem cell research abs || Nucleic acid research abs || Herpes research abs || Bronchitis research abs || Schizophrenia research abs || Tuberculosis research abs || Pneumonia research abs || Constipation research abs || Laxative research abs || hair research abs || hair related research references || testosterone related research references

Asian J Androl. 2002 Dec;4(4):299-301.
Evaluation of androgenic activity of Zingiber officinale and Pentadiplandra brazzeana in male rats.

Kamtchouing P, Mbongue Fandio GY, Dimo T, Jatsa HB.

Laboratoire de Physiologie Animale, Faculte des Sciences, Universite de Yaounde I, Cameroun.

AIM: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. METHODS: The aqueous extracts of the two plants were gavaged separately to 2 groups of rats at a similar dose of 600 mg middot kg(-1) middot day(-1) for 8 days. At the end of the treatment, the animals were killed and the blood, testis, epididymis, seminal vesicles and prostate were collected for biochemical analysis. RESULTS: The aqueous extract of Z. officinale significantly increased in the relative weight of the testis, the serum testosterone level, testicular cholesterol level and epididymal a-glucosidase activity. The aqueous extract of P. brazzeana significantly increased the weights of the testis, seminal vesicles and prostate. It also significantly increased the serum and testicular testosterone level. The fructose, alpha-glucosidase and cholesterol levels in P. brazzeana-treated rats were increased by 28 %, 35 % and 114 %, respectively. CONCLUSION: The aqueous extracts of both P. brazzeana and Z. officinale have an androgenic activity, which seems to be more potent with P. brazzeana than with Z. officinale.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12508133&dopt=Abstract

Biol Pharm Bull. 2002 May;25(5):682-5.
Inhibition of human drug metabolizing cytochrome P450 by buprenorphine.

Umehara K, Shimokawa Y, Miyamoto G.

Department of Drug Metabolism, Drug Safety Research Center, Tokushima Research Institute, Osaka Pharmaceutical Co, Ltd, Kagasuno, Japan. k_umeharesearch.otsuka.co.jp

The effects of buprenorphine, a powerful mixed agonist/antagonist analgesic, on several cytochrome P450 (CYP) isoform specific reactions in human liver microsomes were investigated to predict drug interaction of buprenorphine in vivo from in vitro data. The following eight CYP-catalytic reactions were used in this study: CYPlA1/2-mediated 7-ethoxyresorufin O-deethylation, CYP2A6-mediated coumarin 7-hydroxylation, CYP2B6-mediated 7-benzyloxyresorufin O-debenzylation, CYP2C8/9-mediated tolbutamide methylhydroxylation, CYP2C19-mediated S-mephenytoin 4-hydroxylation, CYP2D6-mediated bufuralol 1'-hydroxylation, CYP2E1-mediated chlorzoxazone 6-hydroxylation, and CYP3A4-mediated testosterone 6beta-hydroxylation. Buprenorphine strongly inhibited the CYP3A4- and CYP2D6-catalyzed reactions with Ki values of 14.7 microM and 21.4 microM, respectively. The analgesic also weakly inhibited specific reactions catalyzed by CYP1A1/2 (Ki=132 microM), CYP2B6 (Ki=133 microM), CYP2C19 (Ki=146 microM), CYP2C8/9 (IC50>300 microM), and CYP2E1 (IC50>300 microM), but not CYP2A6 mediated pathway. In consideration of the Ki values obtained in this study and the therapeutic concentration of buprenorphine in human plasma, buprenorphine would not be predicted to cause clinically significant interactions with other CYP-metabolized drugs.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12033517&dopt=Abstract

BMC Clin Pathol. 2002 May 23;2(1):2.
Effects of EDTA and Sodium Citrate on hormone measurements by fluorometric (FIA) and immunofluorometric (IFMA) methods.

Kohek M, Leme C, Nakamura IT, De Oliveira SA, Lando V, Mendonca BB.

Lab Hormonios e Genetica Molecular/LIM 42, Hospital das Clinicas da FMUSP, Sao Paulo, Brasil. kohesp.br

BACKGROUND: Measurements of hormonal concentrations by immunoassays using fluorescent tracer substance (Eu3+) are susceptible to the action of chemical agents that may cause alterations in its original structure. Our goal was to verify the effect of two types of anticoagulants in the hormone assays performed by fluorometric (FIA) or immunofluorometric (IFMA) methods. METHODS: Blood samples were obtained from 30 outpatients and were drawn in EDTA, sodium citrate, and serum separation Vacutaine&rcircledR;Blood Collection Tubes. Samples were analyzed in automatized equipment AutoDelfiatrade mark (Perkin Elmer Brazil, Wallac, Finland) for the following hormones: Luteinizing hormone (LH), Follicle stimulating homone (FSH), prolactin (PRL), growth hormone (GH), Sex hormone binding globulin (SHBG), thyroid stimulating hormone (TSH), insulin, C peptide, total T3, total T4, free T4, estradiol, progesterone, testosterone, and cortisol. Statistical analysis was carried out by Kruskal-Wallis method and Dunn's test. RESULTS: No significant differences were seen between samples for LH, FSH, PRL and free T4. Results from GH, TSH, insulin, C peptide, SHBG, total T3, total T4, estradiol, testosterone, cortisol, and progesterone were significant different between serum and EDTA-treated samples groups. Differences were also identified between serum and sodium citrate-treated samples in the analysis for TSH, insulin, total T3, estradiol, testosterone and progesterone. CONCLUSIONS: We conclude that the hormonal analysis carried through by FIA or IFMA are susceptible to the effects of anticoagulants in the biological material collected that vary depending on the type of assay.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12033989&dopt=Abstract [PubMed - as supplied by publisher]

J Endocrinol Invest. 2002 May;25(5):455-8.
Testosterone acts as a coronary vasodilator by a calcium antagonistic action.

English KM, Jones RD, Jones TH, Morice AH, Channer KS.

Department of Cardiology, Royal Hallamshire Hospital, Sheffield, UK.

T acts as a vasodilator in vitro and in vivo. Supplemental T therapy in humans with angina improves symptoms and reduces objective measures of ischemia. In left anterior descending coronary arteries taken from adult male Wistar rats, T abolishes 100+/-4.2% of calcium-dependent contraction induced by potassium chloride, 82.3+/-6.1% of the mostly calcium-dependent contraction induced by prostaglandin-F-2-alpha, but only 45.3+/-3.4% of the contraction induced by phorbol-12,13-dibutyrate (PDBu) in the presence of extracellular calcium, and 54.5+/-4.5% of the contraction induced by PDBu in the absence of extracellular calcium. These findings suggest that T is primarily inhibiting the calcium-dependent elements of vascular contraction.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12035943&dopt=Abstract

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It changes personal appearance and identity in social context. Saw palmetto berry extract is a widely known herbfor hair loss as well as BPH problems in Western world. Saw palmetto berry contains phytochemicals that inhibits 5-alpha-reductase that converts testosterone to DHT.

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