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Methods Find Exp Clin Pharmacol. 2002 Apr;24(3):125-30.
Testosterone and estradiol modulate TNF-alpha-induced expression of adhesion molecules in endothelial cells.

Zhang X, Wang L, Dou Y, Zhao J, Jiang T, Qiao Z, Qiao J.

Laboratory of Molecular Biology, Shanxi Medical University, Taiyuan, P.R. China.

Cytokine-activated endothelial expression of adhesion molecules plays an important role in immune responses. In the present study, we investigated the influences of testosterone and 17 beta-estradiol on tumor necrosis factor-alpha (TNF-alpha)-induced expression of adhesion molecules in human umbilical vein endothelial cells (HUVEC). HUVEC were incubated with TNF-alpha, testosterone or 17 beta-estradiol separately, or in a combination of TNF-alpha plus testosterone or 17 beta-estradiol. The expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) was evaluated at 3, 6, 12 and 24 h following exposure by flow cytometric analysis. The results showed that although testosterone or 17 beta-estradiol did not affect the expression of these adhesion molecules in unstimulated HUVEC, both of them transiently increased the expression of E-selectin and VCAM-1 in TNF-alpha stimulated HUVEC. Neither testosterone nor 17 beta-estradiol affected the expression of ICAM-1 induced by TNF-alpha. It is concluded that both testosterone and 17 beta-estradiol increase TNF-alpha-induced expression of E-selectin and VCAM-1 in endothelial cells and these facts might indicate a mechanism by which gonadal hormones can indirectly enhance immune responses.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12087872&dopt=Abstract

J Child Neurol. 2002 Apr;17(4):265-8.
Insulin resistance in epileptic girls who gain weight after therapy with valproic acid.

Verrotti A, Basciani F, De Simone M, Trotta D, Morgese G, Chiarelli F.

Department of Medicine, University of Chieti, Ospedale Policlinico, Italy. averrotbelix.unich.it

Valproic acid is effective for treatment of many types of epilepsy, but its use in epileptic patients can be associated with an increase in body weight that could interfere with treatment compliance. The weight gain may result from different mechanisms, but the exact pathogenesis is still unknown. To evaluate insulin sensitivity in adolescents who gained weight during treatment with valproic acid, we studied 20 girls with different types of epilepsy: 15 patients had primary generalized seizures, including absence seizures (3 cases), and 5 patients had partial seizures. After 1 year of valproic acid treatment, the obese patients had serum insulin levels significantly higher than patients who did not gain weight (51.4 +/- 25.3 versus 28.2 +/- 12.9). Moreover, we observed that epileptic patients who gained weight were also insulin resistant in comparison with nonobese epileptic subjects. At the end of treatment, all patients showed normal levels of serum testosterone, androstenedione, dehydroepiandrosterone sulfate, follicle-stimulating hormone (FSH), and luteinizing hormone. We found no significant correlation between insulinemia and serum valproic acid concentrations in obese and nonobese patients treated with valproic acid. Our study demonstrates that basal hyperinsulinemia and insulin resistance can be present in patients who develop obesity during valproic acid treatment. Therefore, these obese patients could be exposed to the risks related to these metabolic abnormalities; if these data are confirmed in longer studies, these side effects may raise some concerns about the safety of valproic acid.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12088081&dopt=Abstract

Int Urol Nephrol. 1998;30(6):745-53.
Influence of luteinizing hormone-releasing hormone analogues on serum levels of prostatic acid phosphatase and prostatic specific antigen in patients with metastatic carcinoma of the prostate.

Sasagawa I, Kubota Y, Nakada T, Suzuki H, Hirano J, Sugano O, Kato H, Imamura A, Mastushita K, Onmura Y, Saito M, Adachi M.

Department of Urology, Yamagata University, School of Medicine, Japan.

Serum concentrations of luteinizing hormone (LH), testosterone, prostatic acid phosphatase (PAP) and prostatic specific antigen (PSA) were measured in 16 patients with advanced prostatic cancer before and after treatment with luteinizing hormone-releasing hormone (LHRH) analogue. An initial rise of serum LH and testosterone levels was observed on day 2 of the treatment. Subsequently, serum concentrations of PAP and PSA showed a transient increase on day 5 of the treatment. This indicates that LHRH analogues had better be given in combination with antiandrogens in patients with metastatic carcinoma of the prostate.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10195870&dopt=Abstract

Mol Cell Endocrinol. 2002 Jun 28;192(1-2):17-25.
Three mRNA species for mammalian-type gonadotropin-releasing hormone in the brain of the eel Anguilla japonica.

Okubo K, Suetake H, Aida K.

Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, 113-8657, Tokyo, Japan.

A variant mRNA species for mammalian-type gonadotropin-releasing hormone (mGnRH) that retains first intron has recently been found in the brain of a primitive teleost, the eel Anguilla japonica. Here we have found that this mRNA species is generated from an alternative upstream transcription start site of the mGnRH gene. In addition, the upstream start site produces another mRNA species that lacks first intron. Both of these two variant mRNA species, however, have the same open reading frame as the downstream mGnRH mRNA, whose introns are all spliced out, and therefore, all three mGnRH mRNA species expressed in the eel brain would translate a common prepro-mGnRH polypeptide. Quantitative analysis revealed that the upstream mRNAs are less abundant accounting for not more than 1% of the total mGnRH mRNAs. When testosterone was administered (1 mg per fish per day) continuously for 5 days to juvenile male eels, both upstream and downstream mGnRH mRNA species in the brain were up-regulated. However, the level of the upstream mRNAs increased by 3-5-fold after 12 days of the treatment, whereas that of the downstream mRNA showed a more modest increase (about 2-fold) although this increase was more rapid. These results demonstrate that both upstream and downstream mGnRH mRNAs in the eel brain are under the hormonal control, but they are regulated in different and independent manners.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12088863&dopt=Abstract

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Hair Million is an herbal alternative. It is a formula made of traditional, edible herbs and has been anecdotally demonstrated the efficacy to ward off hair loss problems.

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