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Interferon research abs 1 || Hemoglobin research abs || Stem cell research abs || Nucleic acid research abs || Herpes research abs || Bronchitis research abs || Schizophrenia research abs || Tuberculosis research abs

J Infect Dis. 2002 Dec 15;186(12):1797-807. Epub 2002 Nov 19.
Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M. kansasii.

Arend SM, van Meijgaarden KE, de Boer K, de Palou EC, van Soolingen D, Ottenhoff TH, van Dissel JT.

Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. s.m.arenumc.nl

T cell responses to ESAT-6 and culture filtrate protein 10 (CFP-10), antigens expressed by Mycobacterium tuberculosis but not by M. bovis bacille Calmette-Guerin (BCG), were found to discriminate reliably between infection with M. tuberculosis and BCG vaccination. Because the esat-6 and cfp-10 genes occur in M. kansasii and M. marinum, T cell responses to ESAT-6 and CFP-10 were investigated in patients infected with M. kansasii or M. marinum, persons intensively exposed to environmental mycobacteria, and unexposed control subjects. Tuberculin skin tests were performed, and peripheral blood mononuclear cells were cocultured with ESAT-6, CFP-10, peptide mixtures of ESAT-6 and CFP-10, and control antigens. When enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot assay (ELISPOT) were used to measure interferon-gamma production, most M. kansasii- or M. marinum-infected patients and several persons exposed to environmental mycobacteria were found to respond to ESAT-6 and/or CFP-10. ELISA and ELISPOT yielded comparable results, as did whole antigen and peptides (P<.0001). These results may be relevant for the development of novel assays for diagnosis of tuberculosis.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12447766&dopt=Abstract

Ann Trop Paediatr. 2000 Sep;20(3):223-6.
A retrospective review of paediatric lymph node tuberculosis in Port Moresby, Papua New Guinea.

McMaster P, Ezeilo N, Vince JD.

Clinical Sciences Department, Port Moresby General Hospital, Papua New Guinea. Paddy_McMasteompuserve.com

The study aimed to examine the perception that the relapse rate following standard short-course chemotherapy in children with lymph node tuberculosis is greater than in similarly treated children with pulmonary tuberculosis (TB). The treatment records, clinical data and results of investigations of 427 children treated for lymph node TB between 1989 and 1996 were analysed. The results and role of investigations are discussed. The relapse rate was compared with that of 892 children treated for pulmonary TB during the same period. The documented relapse rate for lymph node TB was 2.8% compared with 0.6% in pulmonary TB. This highly significant difference led to a prospective study of outcome of lymph node TB treatment in Port Moresby.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11064776&dopt=Abstract

FEMS Immunol Med Microbiol. 2000 Nov;29(3):203-11.
Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells.

Biswas D, Itoh K, Sasakawa C.

Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan.

Campylobacter jejuni isolates obtained from human and animal sources showed different invasion levels into human embryonic intestinal (INT-407) cells. There was no significant relation between the degree of invasion and cytotoxins production. The depolymerization of both microfilaments by cytochalasin-D and microtubules by colchicine, demecolcine and nocodazole or stabilization of microtubules by paclitaxel reduced the invasiveness of C. jejuni, although microfilament depolymerization showed greater inhibition than microtubule depolymerization. Interference with receptor-mediated endocytosis by G-strophanthin and monodansylcadaverine and inhibition of endosome acidification by monensin reduced the number of viable intracellular C. jejuni cells. Furthermore inhibition of only host protein kinases by staurosporine, but not phosphoinositide 3-kinase by wortmannin or protein kinase-C by calphostin-C, significantly reduced invasion of epithelial cells by C. jejuni. These data suggest that the internalization mechanism triggered by C. jejuni is strikingly different from the microfilament-dependent invasion mechanism exhibited by many of the well-studied enteric bacteria such as enteroinvasive strains of Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersinia enterocolitica and Yersinia pseudotuberculosis.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11064267&dopt=Abstract

FEMS Microbiol Lett. 2000 Nov 15;192(2):263-8.
Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis.

Narayanan S, Selvakumar S, Aarati R, Vasan SK, Narayanan PR.

Department of Immunology, Tuberculosis Research Centre, Mayor V.R. Ramanathan Road, Chennai 600 031, Chetput, India.

Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter.

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11064205&dopt=Abstract

Cardiovasc Pathol. 2000 Sep-Oct;9(5):267-71.
Subvalvular left ventricular aneurysms.

Deshpande J, Vaideeswar P, Sivaraman A.

Department of Pathology, Topiwala National Medical College, Mumbai-400008, India. atharnaahoo.com

Subvalvular left ventricular aneurysms can be subaortic or submitral with variable etiology. This is a retrospective study of 19 subvalvular aneurysms seen in 16 cases. There were 12 isolated subaortic aneurysms, 3 isolated submitral aneurysms, and in 1 case with multiple subaortic and submitral aneurysms. Subaortic aneurysms were associated with infective endocarditis, while there seems to be a strong association between submitral aneurysms and tuberculosis. Five cases of subvalvular aneurysm has associated aneurysm sinus of Valsalva-this association suggests a congenital weakness in the attachment of aortic and mitral annuli to the underlying myocardium. Cardio Pathol 2000;9:267-271

online pharmacy ref. source: www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11064273&dopt=Abstract

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There are a number of approaches to hair loss problems.
Hair Million is an herbal alternative. It is a formula made of traditional, edible herbs and has been anecdotally demonstrated the efficacy to ward off hair loss problems.

There is no singular medical or alternative cure for hair loss since the biology of hair growth is a highly complicated phenomenon. It is unknown how Hair Million stops hair loss, and promotes hair restoration. The advantages of Hair Million over other approaches are, firstly, Hair Million is comparatively inexpensive, and secondly, it is made only of traditionally used safe and healthy herbs that promote hair growth according to Chinese pharmacopoeia. In addition, Hair Million is cardiotonic, meaning that Hair Million consists of herbs that strengthens your heart, according to Chinese medicine. There is an interesting research paper which correlates baldness to heart diseases: people with alopecia or hair loss problems are significantly more likely to develop heart attacks.

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